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. 2013 Jul;15(7):749–760. doi: 10.1593/neo.121956

Figure 6.

Figure 6

Restoration of Sh3gl2 expression inhibits proliferation and migration. (A) Rescue of Sh3gl2 expression in RT4 cells using Sh3gl2-expressing lentivirus was verified by immunoblot analysis. (B, C) Re-expression attenuated proliferation in two independent clones silenced for Sh3gl2, compared to cells expressing an irrelevant gene, LacZ. *P < .05 compared to LacZ at corresponding time point. (D) Reconstitution of Sh3gl2 expression in T24 cells was confirmed by immunoblot analysis. (E) Restoration of Sh3gl2 expression inhibited proliferation, as measured at 48 hours after seeding. *P < .05 compared to proliferation in LacZ-expressing cells. (F) Restoration of Sh3gl2 expression inhibited EGF-stimulated migration in T24 cells. (G) The graph represents average distance migrated, and data are presented as means ± SD. *P < .05 compared to distance migrated in LacZ-expressing cells.