Abstract
Sequence analysis of genomic clones containing the intact Dictyostelium transposable element DIRS-1 reveals that in five of six cases DIRS-1 has inserted into other DIRS-1 sequences. The nucleotide sequences just beyond the endpoints of the terminal repeats of five different genomic clones can be aligned with different regions of the internal nucleotide sequence of DIRS-1. In the three genomic clones which contain flanking sequences on both sides of the element, both flanking sequences are homologous with DIRS-1. In one of these clones, both extended flanking sequences represent the full 4.1-kilobase EcoRI fragment of DIRS-1, which has been interrupted by the insertion of an intact DIRS-1 element. There is no duplication or deletion (except possibly 1 base) of the DIRS-1 sequence upon insertion of a second DIRS-1 transposon. DIRS-1-into-DIRS-1 insertions can occur in either a colinear or inverted orientation with respect to the target sequence; the target sequence need not be an intact DIRS-1 element. We also describe a cDNA clone which could be derived by transcription of a sequence that resulted from a DIRS-1-into-DIRS-1 insertion and discuss its significance concerning the function of the heat-shock promoters found in the terminal repeats of DIRS-1 and in other DIRS-1-related sequences.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Calos M. P., Miller J. H. Transposable elements. Cell. 1980 Jul;20(3):579–595. doi: 10.1016/0092-8674(80)90305-0. [DOI] [PubMed] [Google Scholar]
- Cappello J., Zuker C., Lodish H. F. Repetitive Dictyostelium heat-shock promotor functions in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Apr;4(4):591–598. doi: 10.1128/mcb.4.4.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chung S., Zuker C., Lodish H. F. A repetitive and apparently transposable DNA sequence in Dictyostelium discoideum associated with developmentally regulated RNAs. Nucleic Acids Res. 1983 Jul 25;11(14):4835–4852. doi: 10.1093/nar/11.14.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunsmuir P., Brorein W. J., Jr, Simon M. A., Rubin G. M. Insertion of the Drosophila transposable element copia generates a 5 base pair duplication. Cell. 1980 Sep;21(2):575–579. doi: 10.1016/0092-8674(80)90495-x. [DOI] [PubMed] [Google Scholar]
- Galas D. J., Calos M. P., Miller J. H. Sequence analysis of Tn9 insertions in the lacZ gene. J Mol Biol. 1980 Nov 25;144(1):19–41. doi: 10.1016/0022-2836(80)90213-2. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy E., Löfdahl S. Transposition of Tn554 does not generate a target duplication. Nature. 1984 Jan 19;307(5948):292–294. doi: 10.1038/307292a0. [DOI] [PubMed] [Google Scholar]
- Rosenzweig B., Liao L. W., Hirsh D. Target sequences for the C. elegans transposable element Tc1. Nucleic Acids Res. 1983 Oct 25;11(20):7137–7140. doi: 10.1093/nar/11.20.7137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tu C. P., Cohen S. N. Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell. 1980 Jan;19(1):151–160. doi: 10.1016/0092-8674(80)90396-7. [DOI] [PubMed] [Google Scholar]
- Zuker C., Cappello J., Chisholm R. L., Lodish H. F. A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock. Cell. 1983 Oct;34(3):997–1005. doi: 10.1016/0092-8674(83)90557-3. [DOI] [PubMed] [Google Scholar]
- Zuker C., Cappello J., Lodish H. F., George P., Chung S. Dictyostelium transposable element DIRS-1 has 350-base-pair inverted terminal repeats that contain a heat shock promoter. Proc Natl Acad Sci U S A. 1984 May;81(9):2660–2664. doi: 10.1073/pnas.81.9.2660. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zuker C., Lodish H. F. Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5386–5390. doi: 10.1073/pnas.78.9.5386. [DOI] [PMC free article] [PubMed] [Google Scholar]