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. 1984 Nov;4(11):2449–2454. doi: 10.1128/mcb.4.11.2449

Replication of DNA containing 5-bromouracil can be mutagenic in Syrian hamster cells.

E R Kaufman
PMCID: PMC369076  PMID: 6513925

Abstract

A new protocol for inducing mutations in mammalian cells in culture by exposure to the thymidine analog 5-bromodeoxyuridine (BrdUrd) was established. This protocol, called "DNA-dependent" mutagenesis, involved the incorporation of BrdUrd into DNA under nonmutagenic conditions and the subsequent replication of the 5-bromouracil (BrUra)-containing DNA under mutagenic conditions but with no BrdUrd present in the culture medium. The mutagenic conditions were induced by allowing BrUra-containing DNA to replicate in the presence of high concentrations of thymidine. This generated high intracellular levels of dTTP and dGTP, causing nucleotide pool imbalance. The mutagenesis induced by this protocol was found to correlate with the level of BrUra substituted for thymine in DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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