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. 1984 Nov;4(11):2529–2531. doi: 10.1128/mcb.4.11.2529

Cell cycle phases in the unequal mother/daughter cell cycles of Saccharomyces cerevisiae.

B J Brewer, E Chlebowicz-Sledziewska, W L Fangman
PMCID: PMC369084  PMID: 6392855

Abstract

During cell division in the yeast Saccharomyces cerevisiae mother cells produce buds (daughter cells) which are smaller and have longer cell cycles. We performed experiments to compare the lengths of cell cycle phases in mothers and daughters. As anticipated from earlier indirect observations, the longer cell cycle time of daughter cells is accounted for by a longer G1 interval. The S-phase and the G2-phase are of the same duration in mother and daughter cells. An analysis of five isogenic strains shows that cell cycle phase lengths are independent of cell ploidy and mating type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brewer B. J., Zakian V. A., Fangman W. L. Replication and meiotic transmission of yeast ribosomal RNA genes. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6739–6743. doi: 10.1073/pnas.77.11.6739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caro L. G. Chromosome replication in Escherichia coli. 3. Segregation of chromosomal strands in multi-forked replication. J Mol Biol. 1970 Mar 14;48(2):329–338. doi: 10.1016/0022-2836(70)90164-6. [DOI] [PubMed] [Google Scholar]
  3. Carter B. L., Jagadish M. N. Control of cell division in the yeast Saccharomyces cerevisiae cultured at different growth rates. Exp Cell Res. 1978 Mar 15;112(2):373–383. doi: 10.1016/0014-4827(78)90220-3. [DOI] [PubMed] [Google Scholar]
  4. Carter B. L., Jagadish M. N. The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1978 Mar 1;112(1):15–24. doi: 10.1016/0014-4827(78)90520-7. [DOI] [PubMed] [Google Scholar]
  5. Fangman W. L., Hice R. H., Chlebowicz-Sledziewska E. ARS replication during the yeast S phase. Cell. 1983 Mar;32(3):831–838. doi: 10.1016/0092-8674(83)90069-7. [DOI] [PubMed] [Google Scholar]
  6. Forte M. A., Fangman W. L. Naturally occurring cross-links in yeast chromosomal DNA. Cell. 1976 Jul;8(3):425–431. doi: 10.1016/0092-8674(76)90155-0. [DOI] [PubMed] [Google Scholar]
  7. Hartwell L. H., Unger M. W. Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. J Cell Biol. 1977 Nov;75(2 Pt 1):422–435. doi: 10.1083/jcb.75.2.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  9. Lord P. G., Wheals A. E. Asymmetrical division of Saccharomyces cerevisiae. J Bacteriol. 1980 Jun;142(3):808–818. doi: 10.1128/jb.142.3.808-818.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PUCK T. T., STEFFEN J. LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963 Sep;3:379–397. doi: 10.1016/s0006-3495(63)86828-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rivin C. J., Fangman W. L. Cell cycle phase expansion in nitrogen-limited cultures of Saccharomyces cerevisiae. J Cell Biol. 1980 Apr;85(1):96–107. doi: 10.1083/jcb.85.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Slater M. L., Sharrow S. O., Gart J. J. Cell cycle of Saccharomycescerevisiae in populations growing at different rates. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3850–3854. doi: 10.1073/pnas.74.9.3850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sloat B. F., Pringle J. R. A mutant of yeast defective in cellular morphogenesis. Science. 1978 Jun 9;200(4346):1171–1173. doi: 10.1126/science.349694. [DOI] [PubMed] [Google Scholar]
  14. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  15. Williamson D. H. The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1965 Jun;25(3):517–528. doi: 10.1083/jcb.25.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zakian V. A., Brewer B. J., Fangman W. L. Replication of each copy of the yeast 2 micron DNA plasmid occurs during the S phase. Cell. 1979 Aug;17(4):923–934. doi: 10.1016/0092-8674(79)90332-5. [DOI] [PubMed] [Google Scholar]

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