Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1985 Nov;5(11):3270–3273. doi: 10.1128/mcb.5.11.3270

Binding of anti-Z-DNA antibodies in quiescent and activated lymphocytes: relationship to cell cycle progression and chromatin changes.

L Staiano-Coico, B D Stollar, Z Darzynkiewicz, R Dutkowski, M E Weksler
PMCID: PMC369143  PMID: 3915771

Abstract

Although regions of DNA reacting with anti-Z-DNA antibodies have been identified in the polytene chromosomes of Drosophila spp. and the metaphase chromosomes from a number of different mammalian species, the biological role of this DNA is unknown. Flow cytometry was used in the present studies to quantitate the binding of anti-Z-DNA antibodies in quiescent and activated human peripheral blood lymphocytes; the antibody binding was then correlated with cell cycle phase. The data show that quiescent (G0 or G1Q) lymphocytes are heterogeneous with respect to their reaction with anti-Z-DNA antibodies. The transition from quiescence (G1Q) into the cell cycle (G1), which involves decondensation of chromatin, did not result in any significant change in binding of these antibodies. In contrast, progression of cells from G1 through S and G2 is correlated with a 27% decrease in anti-Z-DNA antibody reactivity relative to total DNA content. No significant change was observed during the transition from G2 to mitosis (M).

Full text

PDF
3270

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arndt-Jovin D. J., Robert-Nicoud M., Zarling D. A., Greider C., Weimer E., Jovin T. M. Left-handed Z-DNA in bands of acid-fixed polytene chromosomes. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4344–4348. doi: 10.1073/pnas.80.14.4344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braylan R. C., Benson N. A., Nourse V., Kruth H. S. Correlated analysis of cellular DNA, membrane antigens and light scatter of human lymphoid cells. Cytometry. 1982 Mar;2(5):337–343. doi: 10.1002/cyto.990020511. [DOI] [PubMed] [Google Scholar]
  3. Darzynkiewicz Z., Dokov V. K., Piénkowski M. Dry mass of lymphocytes during transformation after stimulation by phytohaemagglutinin. Nature. 1967 Jun 17;214(5094):1265–1266. doi: 10.1038/2141265a0. [DOI] [PubMed] [Google Scholar]
  4. Darzynkiewicz Z. Molecular interactions and cellular changes during the cell cycle. Pharmacol Ther. 1983;21(2):143–188. doi: 10.1016/0163-7258(83)90071-2. [DOI] [PubMed] [Google Scholar]
  5. Darzynkiewicz Z., Staiano-Coico L., Melamed M. R. Increased mitochondrial uptake of rhodamine 123 during lymphocyte stimulation. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2383–2387. doi: 10.1073/pnas.78.4.2383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Darzynkiewicz Z., Traganos F., Kapuscinski J., Staiano-Coico L., Melamed M. R. Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry. 1984 Jul;5(4):355–363. doi: 10.1002/cyto.990050411. [DOI] [PubMed] [Google Scholar]
  7. Darzynkiewicz Z., Traganos F., Sharpless T., Melamed M. R. Lymphocyte stimulation: a rapid multiparameter analysis. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2881–2884. doi: 10.1073/pnas.73.8.2881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drew H. R., Dickerson R. E. Conformation and dynamics in a Z-DNA tetramer. J Mol Biol. 1981 Nov 15;152(4):723–736. doi: 10.1016/0022-2836(81)90124-8. [DOI] [PubMed] [Google Scholar]
  9. Lafer E. M., Möller A., Nordheim A., Stollar B. D., Rich A. Antibodies specific for left-handed Z-DNA. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3546–3550. doi: 10.1073/pnas.78.6.3546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lafer E. M., Möller A., Valle R. P., Nordheim A., Rich A., Stollar B. D. Antibody recognition of Z-DNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):155–162. doi: 10.1101/sqb.1983.047.01.020. [DOI] [PubMed] [Google Scholar]
  11. Lancillotti F., Lopez M. C., Alonso C., Stollar B. D. Locations of Z-DNA in polytene chromosomes. J Cell Biol. 1985 May;100(5):1759–1766. doi: 10.1083/jcb.100.5.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leng M., Hartmann B., Malfoy B., Pilet J., Ramstein J., Sage E. Interactions between nucleic acids and antibodies to Z-DNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):163–170. doi: 10.1101/sqb.1983.047.01.021. [DOI] [PubMed] [Google Scholar]
  13. Pardue M. L., Nordheim A., Lafer E. M., Stollar B. D., Rich A. Z-DNA and the polytene chromosome. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):171–176. doi: 10.1101/sqb.1983.047.01.022. [DOI] [PubMed] [Google Scholar]
  14. Pohl F. M., Jovin T. M. Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC). J Mol Biol. 1972 Jun 28;67(3):375–396. doi: 10.1016/0022-2836(72)90457-3. [DOI] [PubMed] [Google Scholar]
  15. Rich A. Right-handed and left-handed DNA: conformational information in genetic material. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):1–12. doi: 10.1101/sqb.1983.047.01.003. [DOI] [PubMed] [Google Scholar]
  16. Robert-Nicoud M., Arndt-Jovin D. J., Zarling D. A., Jovin T. M. Immunological detection of left-handed Z DNA in isolated polytene chromosomes. Effects of ionic strength, pH, temperature and topological stress. EMBO J. 1984 Apr;3(4):721–731. doi: 10.1002/j.1460-2075.1984.tb01875.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stollar B. D., Rezuke W. Separation of anti-histone antibodies from nonimmune histone-precipitating serum proteins, predominantly alpha2-macroglobulin. Arch Biochem Biophys. 1978 Oct;190(2):398–404. doi: 10.1016/0003-9861(78)90292-8. [DOI] [PubMed] [Google Scholar]
  18. Viegas-Péquignot E., Derbin C., Malfoy B., Taillandier E., Leng M., Dutrillaux B. Z-DNA immunoreactivity in fixed metaphase chromosomes of primates. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5890–5894. doi: 10.1073/pnas.80.19.5890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wang A. J., Quigley G. J., Kolpak F. J., van der Marel G., van Boom J. H., Rich A. Left-handed double helical DNA: variations in the backbone conformation. Science. 1981 Jan 9;211(4478):171–176. doi: 10.1126/science.7444458. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES