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. 1985 Nov;5(11):3293–3296. doi: 10.1128/mcb.5.11.3293

DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.

R Ding, K Ghosh, A Eastman, E Bresnick
PMCID: PMC369147  PMID: 3018513

Abstract

Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.

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Selected References

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