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. 1981 May;1(5):387–393. doi: 10.1128/mcb.1.5.387

Linear mitochondrial deoxyribonucleic acid from the yeast Hansenula mrakii.

M Wesolowski 1, H Fukuhara 1
PMCID: PMC369334  PMID: 6965104

Abstract

The mitochondrial deoxyribonucleic acid (mtDNA) from a petite-negative yeast, Hansenula mrakii, was studied. A linear restriction map was constructed with 11 restriction enzymes. The linearity of the genome was confirmed by direct end labeling of the molecule, followed by restriction analysis. The molecular weight of the DNA was found to be 55,000 base pairs. This is the first linear mtDNA found in yeast species. Using specific gene probes obtained from Saccharomyces cerevisiae mtDNA, we have constructed a gene map of H. mrakii mtDNA. The arrangement of genes in this linear genome was very different from the circular mtDNA of other known yeasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Casey J. W., Hsu H. J., Getz G. S., Rabinowitz M. Transfer RNA genes in mitochondrial DNA of grande (wild-type) yeast. J Mol Biol. 1974 Oct 5;88(4):735–747. doi: 10.1016/0022-2836(74)90396-9. [DOI] [PubMed] [Google Scholar]
  2. Cummings D. J., Maki R. A., Conlon P. J., Laping J. Anatomy of mitochondrial DNA from Paramecium aurelia. Mol Gen Genet. 1980;178(3):499–510. doi: 10.1007/BF00337854. [DOI] [PubMed] [Google Scholar]
  3. Faye G., Kujawa C., Fukuhara H. Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae. J Mol Biol. 1974 Sep 5;88(1):185–203. doi: 10.1016/0022-2836(74)90304-0. [DOI] [PubMed] [Google Scholar]
  4. Goddard J. M., Cummings D. J. Structure and replication of mitochondrial DNA from Paramecium aurelia. J Mol Biol. 1975 Oct 5;97(4):593–609. doi: 10.1016/s0022-2836(75)80061-1. [DOI] [PubMed] [Google Scholar]
  5. Goldbach R. W., Borst P., Bollen-de Boer J. E., van Bruggen E. F. The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST. Biochim Biophys Acta. 1978 Nov 21;521(1):169–186. doi: 10.1016/0005-2787(78)90260-5. [DOI] [PubMed] [Google Scholar]
  6. Lewin A., Morimoto R., Rabinowitz M. Restriction enzyme analysis of mitochondrial DNAs of petite mutants of yeast: classification of petites, and deletion mapping of mitochondrial genes. Mol Gen Genet. 1978 Jul 25;163(3):257–275. doi: 10.1007/BF00271955. [DOI] [PubMed] [Google Scholar]
  7. Macino G., Scazzocchio C., Waring R. B., Berks M. M., Davies R. W. Conservation and rearrangement of mitochondrial structural gene sequences. Nature. 1980 Nov 27;288(5789):404–406. doi: 10.1038/288404a0. [DOI] [PubMed] [Google Scholar]
  8. McCarthy B. J., Church R. B. The specificity of molecular hybridization reactions. Annu Rev Biochem. 1970;39:131–150. doi: 10.1146/annurev.bi.39.070170.001023. [DOI] [PubMed] [Google Scholar]
  9. Morimoto R., Merten S., Lewin A., Martin N. C., Rabinowitz M. Physical mapping of genes on yeast mitochondrial DNA: localization of antibiotic resistance loci, and rRNA and tRNA genes. Mol Gen Genet. 1978 Jul 25;163(3):241–255. doi: 10.1007/BF00271954. [DOI] [PubMed] [Google Scholar]
  10. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  11. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  12. Suyama Y., Miura K. Size and structural variations of mitochondrial DNA. Proc Natl Acad Sci U S A. 1968 May;60(1):235–242. doi: 10.1073/pnas.60.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wesolowski M., Fukuhara H. The genetic map of transfer RNA genes of yeast mitochondria: correction and extension. Mol Gen Genet. 1979 Mar 5;170(3):261–275. doi: 10.1007/BF00267059. [DOI] [PubMed] [Google Scholar]
  14. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]

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