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. 1981 May;1(5):418–425. doi: 10.1128/mcb.1.5.418

Cell transformation mediated by chromosomal deoxyribonucleic acid of polyoma virus-transformed cells.

G Della Valle 1, R G Fenton 1, C Basilico 1
PMCID: PMC369337  PMID: 6100965

Abstract

To study the mechanism of deoxyribonucleic acid (DNA)-mediated gene transfer, normal rat cells were transfected with total cellular DNA extracted from polyoma virus-transformed cells. This resulted in the appearance of the transformed phenotype in 1 X 10(-6) to 3 X 10(-6) of the transfected cells. Transformation was invariably associated with the acquisition of integrated viral DNA sequences characteristic of the donor DNA. This was caused not by the integration of free DNA molecules, but by the transfer of large DNA fragments (10 to 20 kilobases) containing linked cellular and viral sequences. Although Southern blot analysis showed that integration did not appear to occur in a homologous region of the recipient chromosome, the frequency of transformation was rather high when compared with that of purified polyoma DNA, perhaps due to "position" effects or to the high efficiency of recombination of large DNA fragments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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