Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1981 Sep;1(9):814–828. doi: 10.1128/mcb.1.9.814

Linkage and evolutionary diversification of developmentally regulated multigene families: tandem arrays of the 401/18 chorion gene pair in silkmoths.

C W Jones 1, F C Kafatos 1
PMCID: PMC369365  PMID: 9279394

Abstract

The coordinately expressed silkmoth chorion genes, 401 and 18, are closely linked as a pair, in divergent orientation. Analysis of overlapping clones (chromosomal "walk") demonstrated that each of the multiple copies of this gene pair is embedded within a larger deoxyribonucleic acid unit, which is tandemly repeated in a few arrays or possibly a single array. Southern analysis and examination of clones from a single individual moth demonstrated that the repeat units are extensively polymorphic in restriction sites, length, and possibly number, no differential amplification was evident during choriogenesis. Intron and 5'-flanking sequences were shown to be specific for the 401/18 gene pair and not to be present elsewhere in the genome. The spatial distribution of variations in the genes and their flanking sequences were examined.

Full text

PDF
814

Images in this article

816Image
on p.816
817Image
on p.817
818Image
on p.818
819Image
on p.819

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  2. Blattner F. R., Blechl A. E., Denniston-Thompson K., Faber H. E., Richards J. E., Slightom J. L., Tucker P. W., Smithies O. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections. Science. 1978 Dec 22;202(4374):1279–1284. doi: 10.1126/science.725603. [DOI] [PubMed] [Google Scholar]
  3. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Efstratiadis A., Crain W. R., Britten R. J., Davidson E. H., Kafatos F. C. DNA sequence organization in the lepidopteran Antheraea pernyi. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2289–2293. doi: 10.1073/pnas.73.7.2289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldsmith M. R., Basehoar G. Organization of the chorion genes of Bombyx mori, a multigene family. I. Evidence for linkage to chromosome 2. Genetics. 1978 Oct;90(2):291–310. doi: 10.1093/genetics/90.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iatrou K., Tsitilou S. G., Goldsmith M. R., Kafatos F. C. Molecular analysis of the GrB mutation in Bombyx mori through the use of chorion cDNA library. Cell. 1980 Jul;20(3):659–669. doi: 10.1016/0092-8674(80)90312-8. [DOI] [PubMed] [Google Scholar]
  8. Jones C. W., Kafatos F. C. Coordinately expressed members of two chorion multi-gene families are clustered, alternating and divergently orientated. Nature. 1980 Apr 17;284(5757):635–638. doi: 10.1038/284635a0. [DOI] [PubMed] [Google Scholar]
  9. Jones C. W., Kafatos F. C. Structure, organization and evolution of developmentally regulated chorion genes in a silkmoth. Cell. 1980 Dec;22(3):855–867. doi: 10.1016/0092-8674(80)90562-0. [DOI] [PubMed] [Google Scholar]
  10. Jones C. W., Rosenthal N., Rodakis G. C., Kafatos F. C. Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell. 1979 Dec;18(4):1317–1332. doi: 10.1016/0092-8674(79)90242-3. [DOI] [PubMed] [Google Scholar]
  11. Kafatos F. C. Recombinant DNA studies on the structure, evolution, and developmental expression of structural gene families. Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1111–1116. doi: 10.4269/ajtmh.1980.29.1111. [DOI] [PubMed] [Google Scholar]
  12. Kafatos F. C., Regier J. C., Mazur G. D., Nadel M. R., Blau H. M., Petri W. H., Wyman A. R., Gelinas R. E., Moore P. B., Paul M. The eggshell of insects: differentiation-specific proteins and the control of their synthesis and accumulation during development. Results Probl Cell Differ. 1977;8:45–145. doi: 10.1007/978-3-540-37332-2_2. [DOI] [PubMed] [Google Scholar]
  13. Kafatos F. C. The cocoonase zymogen cells of silk moths: a model of terminal cell differentiation for specific protein synthesis. Curr Top Dev Biol. 1972;7:125–191. doi: 10.1016/s0070-2153(08)60071-x. [DOI] [PubMed] [Google Scholar]
  14. Kafatos F. C. mRNA stability and cellular differentiation. Acta Endocrinol Suppl (Copenh) 1972;168:319–345. doi: 10.1530/acta.0.071s319. [DOI] [PubMed] [Google Scholar]
  15. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  16. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  17. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  18. Nadel M. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. IV. The chorion proteins of Bombyx mori and their program of synthesis. Dev Biol. 1980 Mar;75(1):26–40. doi: 10.1016/0012-1606(80)90141-4. [DOI] [PubMed] [Google Scholar]
  19. Regier J. C., Kafatos F. C., Kramer K. J., Heinrikson R. L., Keim P. S. Silkmoth chorion proteins. Their diversity, amino acid composition, and the NH-terminal sequence of one component. J Biol Chem. 1978 Feb 25;253(4):1305–1314. [PubMed] [Google Scholar]
  20. Sim G. K., Kafatos F. C., Jones C. W., Koehler M. D., Efstratiadis A., Maniatis T. Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families. Cell. 1979 Dec;18(4):1303–1316. doi: 10.1016/0092-8674(79)90241-1. [DOI] [PubMed] [Google Scholar]
  21. Smith G. P. Evolution of repeated DNA sequences by unequal crossover. Science. 1976 Feb 13;191(4227):528–535. doi: 10.1126/science.1251186. [DOI] [PubMed] [Google Scholar]
  22. Smith G. P. Unequal crossover and the evolution of multigene families. Cold Spring Harb Symp Quant Biol. 1974;38:507–513. doi: 10.1101/sqb.1974.038.01.055. [DOI] [PubMed] [Google Scholar]
  23. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  24. Spradling A. C., Mahowald A. P. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096–1100. doi: 10.1073/pnas.77.2.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Spradling A. C., Waring G. L., Mahowald A. P. Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene. Cell. 1979 Mar;16(3):609–616. doi: 10.1016/0092-8674(79)90034-5. [DOI] [PubMed] [Google Scholar]
  26. Thireos G., Griffin-Shea R., Kafatos F. C. Untranslated mRNA for a chorion protein of Drosophila melanogaster accumulates transiently at the onset of specific gene amplification. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5789–5793. doi: 10.1073/pnas.77.10.5789. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES