Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1981 Sep;1(9):865–870. doi: 10.1128/mcb.1.9.865

Control of initiation and elongation of cilia during ciliary regeneration in Tetrahymena.

G A Hadley 1, N E Williams 1
PMCID: PMC369370  PMID: 9279399

Abstract

Tetrahymena thermophila strain B could regenerate approximately 10% of its somatic ciliary mass in concentrations of cycloheximide believed to block all cytoplasmic protein synthesis. A quantitative study of the relative numbers and lengths of cilia regenerated in the presence and absence of cycloheximide under a variety of conditions suggested that specific initiation and elongation protein factors are involved in the control of ciliary morphogenesis in Tetrahymena.

Full text

PDF
865

Images in this article

867Image
on p.867
867Image
on p.867
867Image
on p.867
867Image
on p.867
869Image
on p.869
869Image
on p.869

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bird R. C., Zimmerman A. M. Induction of tubulin synthesis during cilia regeneration in growing Tetrahymena. Exp Cell Res. 1980 Jul;128(1):199–205. doi: 10.1016/0014-4827(80)90403-6. [DOI] [PubMed] [Google Scholar]
  2. Blum J. J. Existence of a breaking point in cilia and flagella. J Theor Biol. 1971 Nov;33(2):257–263. doi: 10.1016/0022-5193(71)90065-8. [DOI] [PubMed] [Google Scholar]
  3. Frankel J. The effect of nucleic acid antagonists on cell division and oral organelle development in Tetrahymena pyriformis. J Exp Zool. 1965 Jun;159(1):113–147. doi: 10.1002/jez.1401590109. [DOI] [PubMed] [Google Scholar]
  4. Gavin R. H., Frankel J. Macromolecular synthesis, differentiation and cell division in Tetrahymena pyriformis, mating type I variety 1. J Cell Physiol. 1969 Oct;74(2):123–134. doi: 10.1002/jcp.1040740204. [DOI] [PubMed] [Google Scholar]
  5. Guttman S. D., Gorovsky M. A. Cilia regeneration in starved tetrahymena: an inducible system for studying gene expression and organelle biogenesis. Cell. 1979 Jun;17(2):307–317. doi: 10.1016/0092-8674(79)90156-9. [DOI] [PubMed] [Google Scholar]
  6. HAMBURGER K., ZEUTHEN E. Synchronous divisions in Tetrahymena pyriformis as studied in an inorganic medium; the effect of 2,4-dinitrophenol. Exp Cell Res. 1957 Dec;13(3):443–453. doi: 10.1016/0014-4827(57)90074-5. [DOI] [PubMed] [Google Scholar]
  7. Nelsen E. M. Regulation of tubulin during ciliary regeneration in non-growing Tetrahymena. Exp Cell Res. 1975 Aug;94(1):152–158. doi: 10.1016/0014-4827(75)90542-x. [DOI] [PubMed] [Google Scholar]
  8. Rannestad J. The regeneration of cilia in partially deciliated Tetrahymena. J Cell Biol. 1974 Dec;63(3):1009–1017. doi: 10.1083/jcb.63.3.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rodríguez N., Renaud F. L. On the possible role of serotonin in the regulation of regeneration of cilia. J Cell Biol. 1980 May;85(2):242–247. doi: 10.1083/jcb.85.2.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosenbaum J. L., Carlson K. Cilia regeneration in Tetrahymena and its inhibition by colchicine. J Cell Biol. 1969 Feb;40(2):415–425. doi: 10.1083/jcb.40.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ruffolo J. J., Jr Critical point drying of protozoan cells and other biological specimens for scanning electron microscopy: apparatus and methods of specimen preparation. Trans Am Microsc Soc. 1974 Jan;93(1):124–134. [PubMed] [Google Scholar]
  12. Satir B., Sale W. S., Satir P. Membrane renewal after dibucaine deciliation of Tetrahymena. Freeze-fracture technique, cilia, membrane structure. Exp Cell Res. 1976 Jan;97:83–91. doi: 10.1016/0014-4827(76)90657-1. [DOI] [PubMed] [Google Scholar]
  13. Skriver L., Williams N. E. Regeneration of cilia in starved Tetrahymena thermophila involves induced synthesis of ciliary proteins but not synthesis of membrane lipids. Biochem J. 1980 Jun 15;188(3):695–704. doi: 10.1042/bj1880695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Telzer B. R., Rosenbaum J. L. Cell cycle-dependent, in vitro assembly of microtubules onto pericentriolar material of HeLa cells. J Cell Biol. 1979 Jun;81(3):484–497. doi: 10.1083/jcb.81.3.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson G. A., Jr Studies of membrane formation in Tetrahymena pyriformis. I. Rates of phospholipid biosynthesis. Biochemistry. 1967 Jul;6(7):2015–2022. doi: 10.1021/bi00859a020. [DOI] [PubMed] [Google Scholar]
  16. Vaudaux P. E., Williams N. E. Cytoskeletal proteins of the cell surface in Tetrahymena. II. Turnover of major proteins. Exp Cell Res. 1979 Oct 15;123(2):321–331. doi: 10.1016/0014-4827(79)90474-9. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES