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. 2013 Jun 19;29(13):i344–i351. doi: 10.1093/bioinformatics/btt212

Table 1.

Representing the set of possible nucleotides after each flow

Flow No. Flow nucleotide Candidates Remaining sequence Signal
1 A ACGT GCCT 0
2 C CGT GCCT 0
3 G GT GCCT 1
4 T ACT CCT 0
5 A AC CCT 0
6 C C T 2
7 G AGT T 0
8 T AT 1

Note: Here, the wash cycle is ACGT and the sequenced string is GCCT. Initially, any nucleotide is possible—the candidates are ACGT. The first flow (A) produces no incorporation signal; therefore, the candidates for the next nucleotide are CGT. After an incorporation event, as in flows 3 and 6, the candidate nucleotides for the next base in the sequence are all the nucleotides except for the one that was just incorporated.