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. 1992 Apr;12(4):1747–1754. doi: 10.1128/mcb.12.4.1747

Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer.

Y Sekido 1, T Takahashi 1, T P Mäkelä 1, Y Obata 1, R Ueda 1, T Hida 1, K Hibi 1, K Shimokata 1, K Alitalo 1, T Takahashi 1
PMCID: PMC369618  PMID: 1312669

Abstract

The L-myc gene was first isolated from a human small-cell lung cancer (SCLC) cell line on the basis of its amplification and sequence similarity to c-myc and N-myc. A new mechanism of L-myc activation which results from the production of rlf-L-myc fusion protein was recently reported. On the basis of our earlier observation of a rearrangement involving amplified L-myc in an SCLC cell line, ACC-LC-49, we decided to investigate this rearrangement in detail along with the structure of L-myc amplification units in five additional SCLC cell lines. We report here the identification of a novel genomic region, termed jal, which is distinct from rlf and is juxtaposed to and amplified with L-myc during the process of DNA amplification of the region encompassing L-myc. Long-range analysis using pulsed-field gel electrophoresis revealed that the amplified L-myc locus is involved in highly complex intrachromosomal rearrangements with jal and/or rlf. Our results also suggest that the simultaneous presence of rearrangements both in rlf intron 1 and in regions immediately upstream of L-myc may be necessary for the expression of rlf-L-myc chimeric transcripts.

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Selected References

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