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. 1981 Feb;1(2):121–127. doi: 10.1128/mcb.1.2.121

Excision of pyrimidine dimers from nuclear deoxyribonucleic acid in ultraviolet-irradiated Dictyostelium discoideum.

J M Clark 1, R A Deering 1
PMCID: PMC369650  PMID: 6965095

Abstract

A sensitive endonuclease assay was used to study the fate of pyrimidine dimers introduced by ultraviolet irradiation into the nuclear deoxyribonucleic acid of the cellular slime mold Dictyostelium discoideum. Analysis of the frequency of T4 endonuclease V-induced single-strand breaks by alkaline sucrose gradient sedimentation showed that strain NC4 (rad+) removed greater than 98% of the dimers induced by irradiation at 40 J/m2 (254 nm) within 215 min after irradiation. HPS104 (radC44), a mutant sensitive to ultraviolet irradiation, removed 91% under these conditions, although at a significantly slower rate than NC4: only 8% were removed during the 10- to 15-min period immediately after irradiation, whereas NC4 excised 64% during this interval. HPS104 thus appears to be deficient in the activity(ies) responsible for rapidly incising ultraviolet-irradiated nuclear deoxyribonucleic acid at the sites of pyrimidine dimers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakke A. C., Wu J. R., Bonner J. Chromatin structure in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1978 Feb;75(2):705–709. doi: 10.1073/pnas.75.2.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coukell M. B., Cameron A. M. radE, a new radiation-sensitive locus in Dictyostelium discoideum. J Gen Microbiol. 1979 Oct;114(2):247–256. doi: 10.1099/00221287-114-2-247. [DOI] [PubMed] [Google Scholar]
  3. Deering R. A. Dictyostelium discoideum: a valuable eukaryotic system for repair studies. Basic Life Sci. 1975;5B:581–584. doi: 10.1007/978-1-4684-2898-8_24. [DOI] [PubMed] [Google Scholar]
  4. Deering R. A., Jensen D. S. Nuclear and mitochondrial DNA synthesis in gamma ray-resistant and -sensitive slime mold amebas. Biophys J. 1973 Aug;13(8):780–794. doi: 10.1016/S0006-3495(73)86024-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deering R. A., Smith M. S., Thompson B. K., Adolf A. C. Gamma-ray-resistant and -sensitive strains of slime mold (Dictyostelium discoideum). Radiat Res. 1970 Sep;43(3):711–728. [PubMed] [Google Scholar]
  6. Firtel R. A., Bonner J. Characterization of the genome of the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1972 May 28;66(3):339–361. doi: 10.1016/0022-2836(72)90419-6. [DOI] [PubMed] [Google Scholar]
  7. Firtel R. A., Cockburn A., Frankel G., Hershfield V. Structural organization of the genome of Dictyostelium discoideum: analysis by EcoR1 restriction endonuclease. J Mol Biol. 1976 Apr 25;102(4):831–852. doi: 10.1016/0022-2836(76)90294-1. [DOI] [PubMed] [Google Scholar]
  8. Freim J. O., Jr, Deering R. A. Ultraviolet irradiation of the vegetative cells of Dictyostelium discoideum. J Bacteriol. 1970 Apr;102(1):36–42. doi: 10.1128/jb.102.1.36-42.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Friedberg E. C., Clayton D. A. Electron microscopic studies on substrate specificity of T4 excision repair endonuclease. Nature. 1972 May 12;237(5350):99–100. doi: 10.1038/237099a0. [DOI] [PubMed] [Google Scholar]
  10. Friedberg E. C., King J. J. Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. J Bacteriol. 1971 May;106(2):500–507. doi: 10.1128/jb.106.2.500-507.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ganesan A. K. A method for detecting pyrimidine dimers in the DNA of bacteria irradiated with low doses of ultraviolet light. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2753–2756. doi: 10.1073/pnas.70.10.2753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guialis A., Deering R. A. Repair of deoxyribonucleic acid in ultraviolet light-sensitive and -resistant Dictyostelium discoideum strains. J Bacteriol. 1976 Jul;127(1):59–66. doi: 10.1128/jb.127.1.59-66.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Huang A. T., Riddle M. M., Koons L. S. Some properties of a DNA-unwinding protein unique to lymphocytes from chronic lymphocytic leukemia. Cancer Res. 1975 Apr;35(4):981–986. [PubMed] [Google Scholar]
  14. Khoury A. T., Deering R. A. Sedimentation of DNA of Dictyostelium discoideum lysed on alkaline sucrose gradients: role of single-strand breaks in gamma ray lethality of sensitive and resistant strains. J Mol Biol. 1973 Sep 15;79(2):267–284. doi: 10.1016/0022-2836(73)90005-3. [DOI] [PubMed] [Google Scholar]
  15. Kielman J. K., Deering R. A. Ultraviolet light-induced inhibition of cell division and DNA synthesis in axenically grown repair mutants of Dictyostelium discoideum. Photochem Photobiol. 1980 Aug;32(2):149–156. doi: 10.1111/j.1751-1097.1980.tb04002.x. [DOI] [PubMed] [Google Scholar]
  16. LEE H. H., PUCK T. T. The action of ultraviolet radiation on mammalian cells as studied by single-cell techniques. Radiat Res. 1960 Apr;12:340–348. [PubMed] [Google Scholar]
  17. Matthews H. R., Johnson E. M., Steer W. M., Bradbury E. M., Allfrey V. G. The use of netropsin with CsCl gradients for the analysis of DNA and its application to restriction nuclease fragments of ribosomal DNA from Physarum polycephalum. Eur J Biochem. 1978 Jan 16;82(2):569–576. doi: 10.1111/j.1432-1033.1978.tb12052.x. [DOI] [PubMed] [Google Scholar]
  18. Mortelmans K., Friedberg E. C., Slor H., Thomas G., Cleaver J. E. Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2757–2761. doi: 10.1073/pnas.73.8.2757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Richardson C. C. The 5'-terminal nucleotides of T7 bacteriophage deoxyribonucleic acid. J Mol Biol. 1966 Jan;15(1):49–61. doi: 10.1016/s0022-2836(66)80208-5. [DOI] [PubMed] [Google Scholar]
  20. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  22. Tatti K. M., Hudspeth M. E., Johnson P. H., Grossman L. I. Enhancement of buoyant separations between DNA's in preparative CsCl gradients containing distamycin A or netropsin. Anal Biochem. 1978 Sep;89(2):561–571. doi: 10.1016/0003-2697(78)90385-8. [DOI] [PubMed] [Google Scholar]
  23. Unrau P., Wheatcroft R., Cox B., Olive T. The formation of pyrimidine dimers in the DNA of fungi and bacteria. Biochim Biophys Acta. 1973 Jul 27;312(4):626–632. doi: 10.1016/0005-2787(73)90065-8. [DOI] [PubMed] [Google Scholar]
  24. Welker D. L., Deering R. A. In vivo nicking and rejoining of nuclear DNA in ultraviolet-irradiated radiation-resistant and sensitive strains of Dictyostelium discoideum. Mol Gen Genet. 1979 Jan 2;167(3):259–263. doi: 10.1007/BF00267417. [DOI] [PubMed] [Google Scholar]
  25. Welker D. L., Deering R. A. Interactions between radiation-sensitive mutations in double-mutant haploids of Dictyostelium discoideum. Mol Gen Genet. 1979 Jan 2;167(3):265–270. doi: 10.1007/BF00267418. [DOI] [PubMed] [Google Scholar]
  26. Yasuda S., Sekiguchi M. T4 endonuclease involved in repair of DNA. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839–1845. doi: 10.1073/pnas.67.4.1839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zimmer C. Effects of the antibiotics netropsin and distamycin A on the structure and function of nucleic acids. Prog Nucleic Acid Res Mol Biol. 1975;15(0):285–318. doi: 10.1016/s0079-6603(08)60122-1. [DOI] [PubMed] [Google Scholar]

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