Figure 5.

TCR-β analysis in reverted cells shows a diverse repertoire. (A) CD8⁺CD45RA⁻ (memory) subset and the CD8⁺CD45RA⁺CD27⁺ (naïve) were sorted using FACS (>95% purity). Both samples were prepared for high throughput sequencing using a primer set that covers the complete TCR-β repertoire. From both samples approximately 10,000 TCR-β sequences were sequenced using high throughput sequencing. Each clone is depicted as a dot showing its relative frequency to the complete subset (percentage of total reads). As a comparison we used control samples. (B) Vβ-usage of the same samples used in (A) was determined. Use of V-genes (and J-genes, data not shown) was comparable to that of healthy controls (data not shown).