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. 1981 Jul;1(7):594–599. doi: 10.1128/mcb.1.7.594

Cell cycle parameters of Chinese hamster ovary cells during exponential, polyamine-limited growth.

J J Harada 1, D R Morris 1
PMCID: PMC369707  PMID: 9279373

Abstract

We have previously shown that Chinese hamster ovary cells made polyamine deficient by treatment with alpha-methylornithine, an inhibitor of ornithine decarboxylase, grow exponentially in culture at low densities at one-half the rate observed in untreated (control) cultures. In this study, the cell cycle of polyamine-limited cells was examined by using thymidine autoradiography, mitotic index analysis, and fraction labeled mitoses analysis. We found that the longer doubling time of inhibitor-treated cultures was a consequence of increases in the lengths of the G1 and S phases. The expansion of the S phase was proportional to the increase in doubling time (twofold), whereas the G1 phase was lengthened by slightly more than a factor of 2. The lengths of the G2 and M phases were essentially unchanged. Putrescine stimulated the growth of inhibitor-treated cultures and restored the cell cycle parameters to those of untreated cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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