Skip to main content
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1982 Jan;2(1):88–92. doi: 10.1128/mcb.2.1.88

Syntheses and stabilities of proteins related to the polyoma small T antigen in Escherichia coli.

A Horwich, A H Koop, W Eckhart
PMCID: PMC369756  PMID: 6287223

Abstract

We compared the syntheses and turnovers of two proteins related to the polyoma small T antigen synthesized in Escherichia coli from plasmids containing polyoma genomic segments joined to lac control elements. A protein with an authentic polyoma N terminus was more unstable than a protein with N-terminal amino acids derived from beta-galactosidase. Both were more unstable than most bacterial proteins.

Full text

PDF
88

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Horwich A., Koop A. H., Eckhart W. Expression of the gene for the polyoma small T antigen in Escherichia coli. J Virol. 1980 Oct;36(1):125–132. doi: 10.1128/jvi.36.1.125-132.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hunter T., Hutchinson M. A., Eckhart W., Friedmann T., Esty A., LaPorte P., Deininger P. Regions of the polyoma genome coding for T antigens. Nucleic Acids Res. 1979 Dec 20;7(8):2275–2288. doi: 10.1093/nar/7.8.2275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Roberts T. M., Kacich R., Ptashne M. A general method for maximizing the expression of a cloned gene. Proc Natl Acad Sci U S A. 1979 Feb;76(2):760–764. doi: 10.1073/pnas.76.2.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Thummel C. S., Burgess T. L., Tjian R. Properties of simian virus 40 small t antigen overproduced in bacteria. J Virol. 1981 Feb;37(2):683–697. doi: 10.1128/jvi.37.2.683-697.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Thummel C., Tjian R., Grodzicker T. Expression of SV40 T antigen under control of adenovirus promoters. Cell. 1981 Mar;23(3):825–836. doi: 10.1016/0092-8674(81)90447-5. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES