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. 2013 Jul;81(7):2660. doi: 10.1128/IAI.00479-13

Indispensable Role for the Eukaryotic-Like Ankyrin Domains of the Ankyrin B Effector of Legionella pneumophila within Macrophages and Amoebae

Christopher T D Price 1, Souhaila Al-Khodor 1, Tasneem Al-Quadan 1, Yousef Abu Kwaik 1
PMCID: PMC3697590

AUTHOR'S CORRECTION

Volume 78, no. 5, p. 2079–2088, 2010. Page 2084, the legend to Fig. 5B lines 7–15: “(B) Equivalent expression of the AnkB-Cya fusion alleles in L. pneumophila was determined by immunoblot assays of Cya-AnkB fusions expressed in L. pneumophila. Proteins derived from equivalent numbers of bacteria (1 × 108) were loaded onto an SDS-polyacrylamide gel, and Cya fusion proteins were detected on Western blots probed with an anti-M45 antibody, recognizing the N-terminal M45 epitope on all Cya fusions. The blots were reprobed with anti-CAT antibodies, which showed equivalent expression of another protein encoded on the same reporter plasmid.” should read “(B) Equivalent expression of the AnkB-Cya fusion alleles in L. pneumophila was determined by immunoblot assays of Cya-AnkB fusions expressed in L. pneumophila. Proteins derived from equivalent numbers of bacteria (1 × 108) were loaded onto an SDS-polyacrylamide gel, and Cya fusion proteins were detected on a Western blot probed with an anti-M45 antibody, recognizing the N-terminal M45 epitope on all Cya fusions (upper blot). Another blot loaded with proteins derived from an equivalent number of bacteria (1 × 108) per lane was probed with anti-CAT antibodies and showed equivalent expression of the CAT protein, which is encoded on the Cya fusion plasmids (lower blot).”


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