Abstract
The flagellated protozoan Giardia lamblia has been grown only in highly complex media under reduced oxygen tension. Therefore, the organic and physiological requirements for in vitro attachment and short-term (12-h) survival of this organism were determined. In defined maintenance media, a thiol reducing agent (e.g., cysteine) was absolutely required for attachment and survival of this aerotolerant anaerobe. The crude bovine serum Cohn III fraction greatly stimulated attachment and survival. Attachment was decreased at a reduced temperature (24 degrees C as compared with 35.5 degrees C) and absent at 12 degrees C or below. Attachment and survival were strongly dependent upon pH and ionic strength, with optima at pH 6.85 to 7.0 and 200 to 300 mosmol/kg. Sodium chloride was better tolerated than KC1. Reduction of Ca2+ and Mg2+ to below 10(-8) M did not significantly affect attachment.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cobbold P. H. Cytoplasmic free calcium and amoeboid movement. Nature. 1980 Jun 12;285(5765):441–446. doi: 10.1038/285441a0. [DOI] [PubMed] [Google Scholar]
- Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
- Diamond L. S. Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J Parasitol. 1968 Oct;54(5):1047–1056. [PubMed] [Google Scholar]
- Feely D. E., Erlandsen S. L. Isolation and purification of Giardia trophozoites from rat intestine. J Parasitol. 1981 Feb;67(1):59–64. [PubMed] [Google Scholar]
- Feely D. E., Schollmeyer J. V., Erlandsen S. L. Giardia spp.: distribution of contractile proteins in the attachment organelle. Exp Parasitol. 1982 Feb;53(1):145–154. doi: 10.1016/0014-4894(82)90100-x. [DOI] [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Attachment and short-term maintenance of motility and viability of Entamoeba histolytica in a defined medium. J Protozool. 1980 May;27(2):220–225. doi: 10.1111/j.1550-7408.1980.tb04685.x. [DOI] [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Attachment of Entamoeba histolytica to glass in a defined maintenance medium: specific requirement for cysteine and ascorbic acid. J Protozool. 1980 Nov;27(4):474–478. doi: 10.1111/j.1550-7408.1980.tb05402.x. [DOI] [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Clonal growth of Giardia lamblia trophozoites in a semisolid agarose medium. J Parasitol. 1980 Apr;66(2):350–352. [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Entamoeba histolytica and Giardia lamblia: effects of cysteine and oxygen tension on trophozoite attachment to glass and survival in culture media. Exp Parasitol. 1981 Aug;52(1):9–17. doi: 10.1016/0014-4894(81)90055-2. [DOI] [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Entamoeba histolytica and Giardia lamblia: growth responses to reducing agents. Exp Parasitol. 1981 Jun;51(3):382–391. doi: 10.1016/0014-4894(81)90125-9. [DOI] [PubMed] [Google Scholar]
- Grinnell F., Hays D. G. Cell adhesion and spreading factor. Similarity to cold insoluble globulin in human serum. Exp Cell Res. 1978 Aug;115(1):221–229. doi: 10.1016/0014-4827(78)90419-6. [DOI] [PubMed] [Google Scholar]
- Holberton D. V., Ward A. P. Isolation of the cytoskeleton from Giardia. Tubulin and a low-molecular-weight protein associated with microribbon structures. J Cell Sci. 1981 Feb;47:139–166. doi: 10.1242/jcs.47.1.139. [DOI] [PubMed] [Google Scholar]
- McKeehan W. L., McKeehan K. A., Hammond S. L., Ham R. G. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro. 1977 Jul;13(7):399–416. doi: 10.1007/BF02615100. [DOI] [PubMed] [Google Scholar]
- Meyer E. A. Giardia lamblia: isolation and axenic cultivation. Exp Parasitol. 1976 Feb;39(1):101–105. doi: 10.1016/0014-4894(76)90016-3. [DOI] [PubMed] [Google Scholar]
- Owen R. L. The ultrastructural basis of Giardia function. Trans R Soc Trop Med Hyg. 1980;74(4):429–433. doi: 10.1016/0035-9203(80)90043-7. [DOI] [PubMed] [Google Scholar]