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. 1982 May;2(5):479–483. doi: 10.1128/mcb.2.5.479

Massive heat-shock polypeptide synthesis in late chicken embryos: convenient system for study of protein synthesis in highly differentiated organisms.

R Voellmy, P A Bromley
PMCID: PMC369816  PMID: 7110137

Abstract

In cultured eucaryotic cells, heat treatments specifically induced the rapid synthesis of the so-called heat-shock polypeptides. To ascertain the physiological importance of this phenomenon for highly differentiated organisms, we attempted to determine whether the heat-shock response occurs in a living endothermic organism at extreme temperatures, and if so, whether the response is organ specific. We developed a procedure to label proteins efficiently in 5- to 18-day-old chicken embryos. Heat-shock polypeptides of identical sizes of 85,000, 70,000, and 25,000 daltons were synthesized predominantly in chicken embryo fibroblasts and in many different organs of 18-day-old embryos at 42.5 to 44 degrees C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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