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. 1982 Jun;2(6):666–673. doi: 10.1128/mcb.2.6.666

DNA-Mediated Transfer of an RNA Polymerase II Gene: Reversion of the Temperature-Sensitive Hamster Cell Cycle Mutant TsAF8 by Mammalian DNA

C James Ingles 1, Michael Shales 1
PMCID: PMC369842  PMID: 14582161

Abstract

Treatment of the TsAF8 temperature-sensitive (TS) mutant of Syrian hamster BHK-21 cells, with calcium phosphate precipitates of genomic TS+ DNAs from a variety of mammalian cell lines permitted the selection of TS+ colonies at 40°C. TS+ transformation events were distinguished from spontaneous TS+ reversions in experiments in which α-amanitin-sensitive (AmaS) TS+ DNA was used to transform an AmaR derivative of TsAF8 cells and AmaR TS+ DNA was used to transform AmaS TsAF8 cells. In each case it was possible to demonstrate the unselected acquisition of the appropriate AmaS or AmaR phenotype with the selected TS+ allele. Each of these TS+ transformed cell lines when grown at 40°C contained an RNA polymerase II activity with a sensitivity to inhibition by α-amanitin characteristic of the particular DNA used to transform the TS cells, whereas at 34°C the same cells contained a mixture of AmaR and AmaS polymerase II activities. Together, these data provide convincing evidence that the RNA polymerase II gene determining sensitivity to inhibition by α-amanitin can be transferred to TsAF8 cells and that the TS defect in TsAF8 is a polymerase II mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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