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. 1982 Jul;2(7):752–757. doi: 10.1128/mcb.2.7.752

Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages.

K Sakaguchi, B C Lu
PMCID: PMC369856  PMID: 6927791

Abstract

Two forms of DNA polymerase have been studied in the basidiomycete Coprinus. DNA polymerase from basidiocarp tissues at zygotene-pachytene stage has been purified 3,500-fold and defined as DNA polymerase b by virtue of its insensitivity to N-ethylmaleimide and by its low molecular weight (76,000). This enzyme has optimal activity at pH 7.0 to 7.5, at 200 mM KCl, and at 25 degrees C incubation temperature. It can use polycytidylic acid-oligo(dG)12-18 as template primer in addition to homodeoxypolymers. The DNA polymerase a is mainly produced in the exponentially growing mycelium. It is sensitive to N-ethylmaleimide and has a temperature optimum at 35 degrees C. At the premeiotic S phase, activities from both polymerase a and polymerase b are found in cell-free extracts. The b enzyme is the only DNA polymerase produced during meiotic prophase. Its assayable activity exhibits two peaks, one at premeiotic S stage and one at pachytene. It is possible that DNA polymerase b is responsible for pachytene repairs involved in recombination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baril E. F., Scheiner C., Pederson T. A beta-like DNA polymerase activity in the slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3317–3321. doi: 10.1073/pnas.77.6.3317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang L. M. Phylogeny of DNA polymerase-beta. Science. 1976 Mar 19;191(4232):1183–1185. doi: 10.1126/science.769158. [DOI] [PubMed] [Google Scholar]
  3. Fukasawa H., Yamaguchi M., Chou M. Y., Matsumoto H., Matsukage A. Characterization of two DNA polymerases from cauliflower inflorescence. J Biochem. 1980 Apr;87(4):1167–1175. [PubMed] [Google Scholar]
  4. Furukawa Y., Yamada R. H., Kohno M. Presence of two DNA polymerases in Tetrahymena pyriformis. Nucleic Acids Res. 1979 Dec 20;7(8):2387–2398. doi: 10.1093/nar/7.8.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lowe D., Lewis D. DNA polymerase of a basidiomycete fungus, Coprinus cinereus. Biochem J. 1981 Mar 1;193(3):997–1004. doi: 10.1042/bj1930997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lu B. C., Chiu S. M. Genetic recombination of Coprinus. V. Repair synthesis of deoxyribonucleic acid and its relation to meiotic recombination. Mol Gen Genet. 1976 Aug 19;147(2):121–127. doi: 10.1007/BF00267563. [DOI] [PubMed] [Google Scholar]
  7. Lu B. C., Chiu S. M. Meiosis in Coprinus. IX. The influence of premeiotic S-phase arrest and cold temperature on the meiotic cell cycle. J Cell Sci. 1978 Aug;32:21–30. doi: 10.1242/jcs.32.1.21. [DOI] [PubMed] [Google Scholar]
  8. Lu B. C. Genetic Recombination in Coprinus. IV. a Kinetic Study of the Temperature Effect on Recombination Frequency. Genetics. 1974 Oct;78(2):661–677. doi: 10.1093/genetics/78.2.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lu B. C., Jeng D. Y. Meiosis in Coprinus VII. The prekaryogamy S-phase and the postkaryogamy DNA replication in C. lagopus. J Cell Sci. 1975 May;17(3):461–470. doi: 10.1242/jcs.17.3.461. [DOI] [PubMed] [Google Scholar]
  10. Lu B. C. Meiosis in Coprinus: VI. The control of the initiation of meiosis. Can J Genet Cytol. 1974 Mar;16(1):115–164. [PubMed] [Google Scholar]
  11. Schiebel W., Raffael A. Two groups of deoxyribonucleic acid polymerases from Physarum polycephalum classified by differential sensitivity to N-ethylmaleimide, heparin, cytosine arabinoside triphosphate and ethidium bromide: evidence for a beta-like activity. FEBS Lett. 1980 Nov 17;121(1):81–85. doi: 10.1016/0014-5793(80)81271-3. [DOI] [PubMed] [Google Scholar]
  12. Schlabach A., Fridlender B., Bolden A., Weissbach A. DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization. Biochem Biophys Res Commun. 1971 Aug 20;44(4):879–885. doi: 10.1016/0006-291x(71)90793-5. [DOI] [PubMed] [Google Scholar]
  13. Wawra E., Dolejs I. Evidences for the function of DNA polymerase-beta in unscheduled DNA synthesis. Nucleic Acids Res. 1979 Nov 24;7(6):1675–1686. doi: 10.1093/nar/7.6.1675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weissbach A. Eukaryotic DNA polymerases. Annu Rev Biochem. 1977;46:25–47. doi: 10.1146/annurev.bi.46.070177.000325. [DOI] [PubMed] [Google Scholar]

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