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. 1982 Sep;2(9):1115–1117. doi: 10.1128/mcb.2.9.1115

Regulation by vitamin A of envelope cross-linking in cultured keratinocytes derived from different human epithelia.

H Green, F M Watt
PMCID: PMC369904  PMID: 6891021

Abstract

When keratinocytes derived from different squamous epithelia are cultured in the absence of vitamin A, they form cross-linked envelopes during the last stage of terminal differentiation. Addition of the vitamin inhibits envelope formation, but the degree of inhibition is not the same for different keratinocyte subtypes. In the presence of low concentrations of retinyl acetate, conjunctival keratinocytes form virtually no cross-linked envelopes; esophageal and vaginal keratinocytes are less sensitive to the vitamin, and epidermal keratinocytes are the least sensitive. The suppression of cross-linked envelope formation is not associated with a proportional decrease in the concentration of involucrin, a precursor of the envelope, but occurs at the level of cross-linking itself, a process dependent on an increase in the intracellular concentration of calcium ions. Keratinocytes in which spontaneous envelope cross-linking has been prevented by retinyl acetate promptly form cross-linked envelopes if Ca2+ is introduced into the cytoplasm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banks-Schlegel S., Green H. Involucrin synthesis and tissue assembly by keratinocytes in natural and cultured human epithelia. J Cell Biol. 1981 Sep;90(3):732–737. doi: 10.1083/jcb.90.3.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. FELL H. B., MELLANBY E. Metaplasia produced in cultures of chick ectoderm by high vitamin A. J Physiol. 1953 Mar;119(4):470–488. doi: 10.1113/jphysiol.1953.sp004860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuchs E., Green H. Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell. 1981 Sep;25(3):617–625. doi: 10.1016/0092-8674(81)90169-0. [DOI] [PubMed] [Google Scholar]
  5. Green H. The keratinocyte as differentiated cell type. Harvey Lect. 1980;74:101–139. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  8. Lorand L., Weissmann L. B., Epel D. L., Bruner-Lorand J. Role of the intrinsic transglutaminase in the Ca2+-mediated crosslinking of erythrocyte proteins. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4479–4481. doi: 10.1073/pnas.73.12.4479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rheinwald J. G., Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975 Nov;6(3):331–343. doi: 10.1016/s0092-8674(75)80001-8. [DOI] [PubMed] [Google Scholar]
  10. Rice R. H., Green H. Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions. Cell. 1979 Nov;18(3):681–694. doi: 10.1016/0092-8674(79)90123-5. [DOI] [PubMed] [Google Scholar]
  11. Rothblat G. H., Arbogast L. Y., Ouellette L., Howard B. V. Preparation of delipidized serum protein for use in cell culture systems. In Vitro. 1976 Aug;12(8):554–557. doi: 10.1007/BF02797438. [DOI] [PubMed] [Google Scholar]
  12. Siefring G. E., Jr, Apostol A. B., Velasco P. T., Lorand L. Enzymatic basis for the Ca2+-induced cross-linking of membrane proteins in intact human erythrocytes. Biochemistry. 1978 Jun 27;17(13):2598–2604. doi: 10.1021/bi00606a022. [DOI] [PubMed] [Google Scholar]
  13. Smith F. R., Goodman D. S. The effects of diseases of the liver, thyroid, and kidneys on the transport of vitamin A in human plasma. J Clin Invest. 1971 Nov;50(11):2426–2436. doi: 10.1172/JCI106741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spiegelman B. M., Penningroth S. M., Kirschner M. W. Turnover of tubulin and the N site GTP in Chinese hamster ovary cells. Cell. 1977 Nov;12(3):587–600. doi: 10.1016/0092-8674(77)90259-8. [DOI] [PubMed] [Google Scholar]
  15. Sun T. T., Green H. Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature. 1977 Oct 6;269(5628):489–493. doi: 10.1038/269489a0. [DOI] [PubMed] [Google Scholar]
  16. Watt F. M., Green H. Involucrin synthesis is correlated with cell size in human epidermal cultures. J Cell Biol. 1981 Sep;90(3):738–742. doi: 10.1083/jcb.90.3.738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yaar M., Stanley J. R., Katz S. I. Retinoic acid delays the terminal differentiation of keratinocytes in suspension culture. J Invest Dermatol. 1981 May;76(5):363–366. doi: 10.1111/1523-1747.ep12520026. [DOI] [PubMed] [Google Scholar]

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