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. 1982 Oct;2(10):1212–1219. doi: 10.1128/mcb.2.10.1212

Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.

S J Silverman, M Rose, D Botstein, G R Fink
PMCID: PMC369920  PMID: 6817079

Abstract

The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.

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Selected References

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