Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1982 May;69(5):1119–1125. doi: 10.1172/JCI110547

Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique.

C N Sadur, R H Eckel
PMCID: PMC370176  PMID: 7040473

Abstract

The role of insulin in the regulation of adipose tissue lipoprotein lipase activity in humans was investigated in 11 normal subjects and compared with the effects of 0.9% saline infusions in five control subjects. After a basal adipose tissue biopsy for lipoprotein lipase activity, insulin was rapidly infused to achieve and maintain serum levels of approximately 70 microunits/ml while plasma glucose was kept at basal concentrations. Free fatty acids in serum fell to 27 +/- 3% of basal by 20 min (t = 5.19, P less than 0.001) and triglycerides decreased to 77 +/- 3% of basal by 80 min (t = 3.76, P less than 0.01). Adipose tissue lipoprotein lipase activity failed to increase significantly above that measured in controls by the first 3 h of the study. By 6 h of the infusion a stimulatory effect of insulin on adipose tissue lipoprotein lipase was found (t = 3.94, P less than 0.01). There was no relationship between the amount of glucose infused and the insulin effect on the enzyme. The increase in adipose tissue lipoprotein lipase activity at 6 h, however, was inversely related to the basal lipase activity (r = -0.690, P less than 0.02). Thus, insulin appears to stimulate adipose tissue lipoprotein lipase activity in humans. This effect of insulin is delayed when compared with antilipolysis and the fall in plasma triglyceride. The inverse relationship between insulin-stimulated adipose tissue lipoprotein lipase activity and basal enzyme activity suggests that adipose tissue itself is the main regulator of the lipase response to insulin.

Full text

PDF
1119

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashby P., Robinson D. S. Effects of insulin, glucocorticoids and adrenaline on the activity of rat adipose-tissue lipoprotein lipids. Biochem J. 1980 Apr 15;188(1):185–192. doi: 10.1042/bj1880185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Belfrage P., Vaughan M. Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. J Lipid Res. 1969 May;10(3):341–344. [PubMed] [Google Scholar]
  3. Borensztajn J., Samols D. R., Rubenstein A. H. Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue. Am J Physiol. 1972 Dec;223(6):1271–1275. doi: 10.1152/ajplegacy.1972.223.6.1271. [DOI] [PubMed] [Google Scholar]
  4. Brunzell J. D., Porte D., Jr, Bierman E. L. Abnormal lipoprotein-lipase-mediated plasma triglyceride removal in untreated diabetes mellitus associated with hypertriglyceridemia. Metabolism. 1979 Sep;28(9):901–907. doi: 10.1016/0026-0495(79)90089-1. [DOI] [PubMed] [Google Scholar]
  5. Chen Y. D., Howard J., Huang V., Kraemer F. B., Reaven G. M. Dissociation between plasma triglyceride concentration and tissue lipoprotein lipase deficiency in insulin-deficient rats. Diabetes. 1980 Aug;29(8):643–647. doi: 10.2337/diab.29.8.643. [DOI] [PubMed] [Google Scholar]
  6. Chlouverakis C., Hojnicki D. A modified radiochemical assay for serum free fatty acid determination. Clin Chim Acta. 1974 Jul 15;54(1):91–93. doi: 10.1016/0009-8981(74)90046-1. [DOI] [PubMed] [Google Scholar]
  7. Desbuquois B., Aurbach G. D. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab. 1971 Nov;33(5):732–738. doi: 10.1210/jcem-33-5-732. [DOI] [PubMed] [Google Scholar]
  8. Eckel R. H., Fujimoto W. Y., Brunzell J. D. Insulin regulation of lipoprotein lipase in cultured 3T3-L1 cells. Biochem Biophys Res Commun. 1978 Oct 30;84(4):1069–1075. doi: 10.1016/0006-291x(78)91692-3. [DOI] [PubMed] [Google Scholar]
  9. Garfinkel A. G., Nilsson-ehle P., Schotz M. C. Regulation of lipoprotein lipase. Induction by insulin. Biochim Biophys Acta. 1976 Feb 23;424(2):264–273. doi: 10.1016/0005-2760(76)90194-6. [DOI] [PubMed] [Google Scholar]
  10. Goldberg A. P., Chait A., Brunzell J. D. Postprandial adipose tissue lipoprotein lipase activity in primary hypertriglyceridemia. Metabolism. 1980 Mar;29(3):223–229. doi: 10.1016/0026-0495(80)90063-3. [DOI] [PubMed] [Google Scholar]
  11. Havel R. J., Kane J. P., Balasse E. O., Segel N., Basso L. V. Splanchnic metabolism of free fatty acids and production of triglycerides of very low density lipoproteins in normotriglyceridemic and hypertriglyceridemic humans. J Clin Invest. 1970 Nov;49(11):2017–2035. doi: 10.1172/JCI106422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hollenberg C. H. The origin and glyceride distribution of fatty acids in rat adipose tissue. J Clin Invest. 1966 Feb;45(2):205–216. doi: 10.1172/JCI105333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Insel P. A., Liljenquist J. E., Tobin J. D., Sherwin R. S., Watkins P., Andres R., Berman M. Insulin control of glucose metabolism in man: a new kinetic analysis. J Clin Invest. 1975 May;55(5):1057–1066. doi: 10.1172/JCI108006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Keys A., Fidanza F., Karvonen M. J., Kimura N., Taylor H. L. Indices of relative weight and obesity. J Chronic Dis. 1972 Jul 1;25(6):329–343. doi: 10.1016/0021-9681(72)90027-6. [DOI] [PubMed] [Google Scholar]
  15. Lopes-Virella M. F., Stone P., Ellis S., Colwell J. A. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem. 1977 May;23(5):882–884. [PubMed] [Google Scholar]
  16. Nikkilä E. A., Huttunen J. K., Ehnholm C. Postheparin plasma lipoprotein lipase and hepatic lipase in diabetes mellitus. Relationship to plasma triglyceride metabolism. Diabetes. 1977 Jan;26(1):11–21. doi: 10.2337/diab.26.1.11. [DOI] [PubMed] [Google Scholar]
  17. Nilsson-Ehle P., Carlström S., Belfrage P. Rapid effect on lipoprotein lipase activity in adipose tissue of humans after carbohydrate and lipid intake. Scand J Clin Lab Invest. 1975 Jul;35(4):373–378. [PubMed] [Google Scholar]
  18. Passon P. G., Peuler J. D. A simplified radiometric assay for plasma norepinephrine and epinephrine. Anal Biochem. 1973 Feb;51(2):618–631. doi: 10.1016/0003-2697(73)90517-4. [DOI] [PubMed] [Google Scholar]
  19. Pykälistö O. J., Smith P. H., Brunzell J. D. Determinants of human adipose tissue lipoprotein lipase. Effect of diabetes and obesity on basal- and diet-induced activity. J Clin Invest. 1975 Nov;56(5):1108–1117. doi: 10.1172/JCI108185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem. 1973 Dec;19(12):1350–1356. [PubMed] [Google Scholar]
  21. Rowe J. W., Young J. B., Minaker K. L., Stevens A. L., Pallotta J., Landsberg L. Effect of insulin and glucose infusions on sympathetic nervous system activity in normal man. Diabetes. 1981 Mar;30(3):219–225. doi: 10.2337/diab.30.3.219. [DOI] [PubMed] [Google Scholar]
  22. Scow R. O., Blanchette-Mackie E. J., Smith L. C. Transport of lipid across capillary endothelium. Fed Proc. 1980 Jul;39(9):2610–2617. [PubMed] [Google Scholar]
  23. Spooner P. M., Chernick S. S., Garrison M. M., Scow R. O. Insulin regulation of lipoprotein lipase activity and release in 3T3-L1 adipocytes. Separation and dependence of hormonal effects on hexose metabolism and synthesis of RNA and protein. J Biol Chem. 1979 Oct 25;254(20):10021–10029. [PubMed] [Google Scholar]
  24. Taskinen M. R., Nikkilä E. A. Lipoprotein lipase activity in adipose tissue and in postheparin plasma in human obesity. Acta Med Scand. 1977;202(5):399–408. doi: 10.1111/j.0954-6820.1977.tb16851.x. [DOI] [PubMed] [Google Scholar]
  25. Taskinen M. R., Nikkilä E. A. Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes. Relation to high-density and very-low-density lipoproteins and response to treatment. Diabetologia. 1979 Dec;17(6):351–356. doi: 10.1007/BF01236268. [DOI] [PubMed] [Google Scholar]
  26. Taylor K. G., Galton D. J., Holdsworth G. Insulin-independent diabetes: a defect in the activity of lipoprotein lipase in adipose tissue. Diabetologia. 1979 May;16(5):313–317. doi: 10.1007/BF01223620. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES