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. 1982 Jul;70(1):82–88. doi: 10.1172/JCI110606

In vitro parasite-monocyte interactions in human leishmaniasis. Evidence for an active role of the parasite in attachment.

D J Wyler
PMCID: PMC370229  PMID: 7085890

Abstract

Leishmania are obligate intracellular parasites of mononuclear phagocytes in the mammalian host. To more clearly define features of the early events in host-parasite interaction, human monocytes were so-cultured with Leishmania tropica amastigotes in vitro. Infection of monocytes was time dependent and inhibited at 4 degrees C and in the presence of cytochalasin B. Pretreatment of amastigotes with cytochalasins prevented their attachment to normal monocytes. Untreated amastigotes attached normally but could not enter cytochalasin-pretreated monocytes. This suggests that amastigotes actively participate in attachment but require host cell participation for interiorization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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