Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1982 Oct;70(4):747–751. doi: 10.1172/JCI110670

Generation of leukotrienes by purified human lung mast cells.

D W MacGlashan Jr, R P Schleimer, S P Peters, E S Schulman, G K Adams 3rd, H H Newball, L M Lichtenstein
PMCID: PMC370282  PMID: 7119113

Abstract

Although mediator release from mast cells and basophils plays a central role in the pathogenesis of human allergic disease, biochemical studies have been restricted to rat peritoneal mast cells and basophilic leukemia cells because they could be easily purified. We have used two new techniques of cell separation to purify human lung mast cells to 98% homogeneity. Lung cell suspensions were obtained by dispersion of chopped lung tissue with proteolytic enzymes. Mast cells were then purified from the suspensions by countercurrent centrifugal elutriation and affinity chromatography. The purified mast cells released both histamine and slow-reacting substance of anaphylaxis (SRS-A) (leukotriene C and D) during stimulation with goat anti-human IgE antibody. Moreover, these preparations were able to generate significant quantities of SRS-A (32 +/- 7 x 10(-17) LTD mole-equivalents/mast cell) at all stages of purification, indicating that a secondary cell is not necessary for the antigen-induced release of SRS.

Full text

PDF
747

Images in this article

748Image
on p.748

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. K., Brashler J. R. Ionophore A 23187-induced production of slow reacting substance of anaphylaxis (SRS-A) by rat peritoneal cells in vitro: evidence for production by mononuclear cells. J Immunol. 1978 Mar;120(3):998–1005. [PubMed] [Google Scholar]
  2. Dahlén S. E., Hedqvist P., Hammarström S., Samuelsson B. Leukotrienes are potent constrictors of human bronchi. Nature. 1980 Dec 4;288(5790):484–486. doi: 10.1038/288484a0. [DOI] [PubMed] [Google Scholar]
  3. LICHTENSTEIN L. M., OSLER A. G. STUDIES ON THE MECHANISMS OF HYPERSENSITIVITY PHENOMENA. IX. HISTAMINE RELEASE FROM HUMAN LEUKOCYTES BY RAGWEED POLLEN ANTIGEN. J Exp Med. 1964 Oct 1;120:507–530. doi: 10.1084/jem.120.4.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lewis R. A., Holgate S. T., Roberts L. J., 2nd, Oates J. A., Austen K. F. Preferential generation of prostaglandin D2 by rat and human mast cells. Kroc Found Ser. 1981;14:239–254. [PubMed] [Google Scholar]
  5. Lewis R. A., Wasserman S. I., Goetzi E. J., Austen K. F. Formation of slow-reacting substance of anaphylaxis in human lung tissue and cells before release. J Exp Med. 1974 Nov 1;140(5):1133–1146. doi: 10.1084/jem.140.5.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lichtenstein L. M. The immediate allergic response: in vitro separation of antigen activation, decay and histamine release. J Immunol. 1971 Oct;107(4):1122–1130. [PubMed] [Google Scholar]
  7. MacGlashan D. W., Jr, Lichtenstein L. M. The purification of human basophils. J Immunol. 1980 May;124(5):2519–2521. [PubMed] [Google Scholar]
  8. MacGlashan D. W., Jr, Lichtenstein L. M. The transition from specific to nonspecific desensitization in human basophils. J Immunol. 1981 Dec;127(6):2410–2414. [PubMed] [Google Scholar]
  9. Orange R. P., Moore E. G., Gelfand E. W. The formation and release of slow reacting substance of anaphylaxis (SRS-A) by rat and mouse peritoneal mononuclear cells induced by ionophore A23187. J Immunol. 1980 May;124(5):2264–2267. [PubMed] [Google Scholar]
  10. Paterson N. A., Wasserman S. I., Said J. W., Austen K. F. Release of chemical mediators from partially purified human lung mast cells. J Immunol. 1976 Oct;117(4):1356–1362. [PubMed] [Google Scholar]
  11. Pretlow T. G., 2nd, Pretlow T. P. Centrifugal elutriation (counterstreaming centrifugation) of cells. Cell Biophys. 1979 Jun;1(2):195–210. doi: 10.1007/BF02781351. [DOI] [PubMed] [Google Scholar]
  12. Sheard P., Killingback P. G., Blair A. M. Antigen induced release of histamine and SRS-A from human lung passively sensitized with reaginic serum. Nature. 1967 Oct 21;216(5112):283–284. doi: 10.1038/216283a0. [DOI] [PubMed] [Google Scholar]
  13. Siraganian R. P. An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal Biochem. 1974 Feb;57(2):383–394. doi: 10.1016/0003-2697(74)90093-1. [DOI] [PubMed] [Google Scholar]
  14. Yecies L. D., Wedner H. J., Johnson S. M., Jakschik B. A., Parker C. W. Slow reacting substance (SRS) from ionophore A23187-stimulated peritoneal mast cells of the normal rat. I. Conditions of generation and initial characterization. J Immunol. 1979 May;122(5):2083–2089. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES