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. 1982 Dec;70(6):1225–1233. doi: 10.1172/JCI110721

Effect of somatostatin-induced suppression of postprandial insulin response upon the hypertriglyceridemia associated with a high carbohydrate diet.

H N Ginsberg, A Jacobs, N A Le, J Sandler
PMCID: PMC370339  PMID: 6129260

Abstract

In an attempt to define the relationship between plasma insulin and triglyceride concentrations, we have studied the effect of suppression of the postprandial insulin response upon the secretion and plasma concentration of very low density lipoprotein (VLDL)-triglycerides. Eight nondiabetic subjects with a wide range of fasting plasma triglyceride levels (100-358 mg/dl) were studied during three dietary periods: base line, high carbohydrate (80% calories), and high carbohydrate (80% calories) with a daily intravenous infusion of somatostatin (SRIF) (1.3 micrograms/min) between 800 and 2,100 h. The significant increase in postprandial insulin response observed during high carbohydrate vs. base line was completely abolished during high carbohydrate-SRIF. However, plasma triglyceride levels rose in all subjects during each high carbohydrate period (with/without SRIF) vs. base line and the mean values reached during each period were the same (476 +/- 165 vs. 482 +/- 152 mg/dl, respectively). The secretion of VLDL-triglyceride into plasma was higher in four subjects, the same in two subjects, and lower in one subject during high carbohydrate-SRIF vs. high carbohydrate alone. The mean production rate of VLDL-triglyceride (mg/kg per h) was 25.6 +/- 4.9 during the high carbohydrate and 40.9 +/- 28.1 during the high carbohydrate-SRIF periods. These values were not significantly different. Postprandial glucose levels were slightly increased during high carbohydrate-SRIF, but overnight glucose concentrations were not affected. Plasma FFA levels were not different during the two high carbohydrate periods. Plasma glucagon levels did not appear to affect the results either. This study indicates that postprandial hyperinsulinemia during a high carbohydrate diet is not necessary for induction of hypertriglyceridemia.

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Selected References

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  1. Adams P. W., Kissebah A. H., Harrigan P., Stokes T., Wynn V. The kinetics of plasma free fatty acid and triglyceride transport in patients with idiopathic hypertriglyceridaemia and their relation to carbohydrate metabolism. Eur J Clin Invest. 1974 Jun;4(3):149–161. doi: 10.1111/j.1365-2362.1974.tb00386.x. [DOI] [PubMed] [Google Scholar]
  2. Basso L. V., Havel R. J. Hepatic metabolism of free fatty acids in normal and diabetic dogs. J Clin Invest. 1970 Mar;49(3):537–547. doi: 10.1172/JCI106264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bierman E. L. Insulin and hypertriglyceridemia. Isr J Med Sci. 1972 Mar;8(3):303–308. [PubMed] [Google Scholar]
  4. Blackard W. G., Nelson N. C., Andrews S. S. Portal and peripheral vein immunoreactive glucagon concentrations after arginine or glucose infusions. Diabetes. 1974 Mar;23(3):199–202. doi: 10.2337/diab.23.3.199. [DOI] [PubMed] [Google Scholar]
  5. Desbuquois B., Aurbach G. D. Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab. 1971 Nov;33(5):732–738. doi: 10.1210/jcem-33-5-732. [DOI] [PubMed] [Google Scholar]
  6. Drenick E. J., Brickman A. S., Gold E. M. Dissociation of the obesity-hyperinsulinism relationship following dietary restriction and hyperalimentation. Am J Clin Nutr. 1972 Aug;25(8):746–755. doi: 10.1093/ajcn/25.8.746. [DOI] [PubMed] [Google Scholar]
  7. Eaton B. P., Nye W. H. The relationship between insulin secretion and triglyceride concentration in endogenous lipemia. J Lab Clin Med. 1973 May;81(5):682–695. [PubMed] [Google Scholar]
  8. Farquhar J. W., Frank A., Gross R. C., Reaven G. M. Glucose, insulin, and triglyceride responses to high and low carbohydrate diets in man. J Clin Invest. 1966 Oct;45(10):1648–1656. doi: 10.1172/JCI105472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenfield M., Kolterman O., Olefsky J., Reaven G. M. Mechanism of hypertriglyceridaemia in diabetic patients with fasting hyperglycaemia. Diabetologia. 1980 Jun;18(6):441–446. doi: 10.1007/BF00261698. [DOI] [PubMed] [Google Scholar]
  10. Grundy S. M., Mok H. Y., Zech L., Steinberg D., Berman M. Transport of very low density lipoprotein triglycerides in varying degrees of obesity and hypertriglyceridemia. J Clin Invest. 1979 Jun;63(6):1274–1283. doi: 10.1172/JCI109422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Havel R. J., Kane J. P., Balasse E. O., Segel N., Basso L. V. Splanchnic metabolism of free fatty acids and production of triglycerides of very low density lipoproteins in normotriglyceridemic and hypertriglyceridemic humans. J Clin Invest. 1970 Nov;49(11):2017–2035. doi: 10.1172/JCI106422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heimberg M., Weinstein I., Kohout M. The effects of glucagon, dibutyryl cyclic adenosine 3',5'-monophosphate, and concentration of free fatty acid on hepatic lipid metabolism. J Biol Chem. 1969 Oct 10;244(19):5131–5139. [PubMed] [Google Scholar]
  13. McGarry J., Wright P. H., Foster D. W. Hormonal control of ketogenesis. Rapid activation of hepatic ketogenic capacity in fed rats by anti-insulin serum and glucagon. J Clin Invest. 1975 Jun;55(6):1202–1209. doi: 10.1172/JCI108038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Melish J., Le N. A., Ginsberg H., Steinberg D., Brown W. V. Dissociation of apoprotein B and triglyceride production in very-low-density lipoproteins. Am J Physiol. 1980 Nov;239(5):E354–E362. doi: 10.1152/ajpendo.1980.239.5.E354. [DOI] [PubMed] [Google Scholar]
  15. Nikkilä E. A., Huttunen J. K., Ehnholm C. Postheparin plasma lipoprotein lipase and hepatic lipase in diabetes mellitus. Relationship to plasma triglyceride metabolism. Diabetes. 1977 Jan;26(1):11–21. doi: 10.2337/diab.26.1.11. [DOI] [PubMed] [Google Scholar]
  16. Noma A., Okabe H., Kita M. A new colorimetric micro-determination of free fatty acids in serum. Clin Chim Acta. 1973 Feb 12;43(3):317–320. doi: 10.1016/0009-8981(73)90468-3. [DOI] [PubMed] [Google Scholar]
  17. Olefsky J. M., Farquhar J. W., Reaven G. M. Reappraisal of the role of insulin in hypertriglyceridemia. Am J Med. 1974 Oct;57(4):551–560. doi: 10.1016/0002-9343(74)90006-0. [DOI] [PubMed] [Google Scholar]
  18. Olefsky J., Reaven G. M., Farquhar J. W. Effects of weight reduction on obesity. Studies of lipid and carbohydrate metabolism in normal and hyperlipoproteinemic subjects. J Clin Invest. 1974 Jan;53(1):64–76. doi: 10.1172/JCI107560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Quarfordt S. H., Frank A., Shames D. M., Berman M., Steinberg D. Very low density lipoprotein triglyceride transport in type IV hyperlipoproteinemia and the effects of carbohydrate-rich diets. J Clin Invest. 1970 Dec;49(12):2281–2297. doi: 10.1172/JCI106448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reaven E. P., Reaven G. M. Mechanisms for development of diabetic hypertriglyceridemia in streptozotocin-treated rats. Effect of diet and duration of insulin deficiency. J Clin Invest. 1974 Nov;54(5):1167–1178. doi: 10.1172/JCI107860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reaven G. M., Lerner R. L., Stern M. P., Farquhar J. W. Role of insulin in endogenous hypertriglyceridemia. J Clin Invest. 1967 Nov;46(11):1756–1767. doi: 10.1172/JCI105666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schlierf G., Dorow E. Diurnal patterns of triglycerides, free fatty acids, blood sugar, and insulin during carbohydrate-induction in man and their modification by nocturnal suppression of lipolysis. J Clin Invest. 1973 Mar;52(3):732–740. doi: 10.1172/JCI107235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tobey T. A., Greenfield M., Kraemer F., Reaven G. M. Relationship between insulin resistance, insulin secretion, very low density lipoprotein kinetics, and plasma triglyceride levels in normotriglyceridemic man. Metabolism. 1981 Feb;30(2):165–171. doi: 10.1016/0026-0495(81)90167-0. [DOI] [PubMed] [Google Scholar]
  24. Topping D. L., Mayes P. A. The immediate effects of insulin and fructose on the metabolism of the perfused liver. Changes in lipoprotein secretion, fatty acid oxidation and esterification, lipogenesis and carbohydrate metabolism. Biochem J. 1972 Jan;126(2):295–311. doi: 10.1042/bj1260295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tulloch B. R., Dyal K., Fraser T. R. Increased lipid synthesis by liver slice in a superfusion system following raised glucose or insulin concentration. Diabetologia. 1972 Aug;8(4):267–273. doi: 10.1007/BF01225570. [DOI] [PubMed] [Google Scholar]
  26. Tzagournis M., Chiles R., Ryan J. M., Skillman T. G. Interrelationships of hyperinsulinism and hypertriglyceridemia in young patients with coronary heart disease. Circulation. 1968 Dec;38(6):1156–1163. doi: 10.1161/01.cir.38.6.1156. [DOI] [PubMed] [Google Scholar]
  27. Vale W., Rivier C., Brown M. Regulatory peptides of the hypothalamus. Annu Rev Physiol. 1977;39:473–527. doi: 10.1146/annurev.ph.39.030177.002353. [DOI] [PubMed] [Google Scholar]
  28. Van Tol A. Hypertriglyceridemia in the diabetic rat. Defective removal of serum very low density. Atherosclerosis. 1977 Jan;26(1):117–128. doi: 10.1016/0021-9150(77)90146-0. [DOI] [PubMed] [Google Scholar]
  29. Wahren J. Influence of somatostatin on carbohydrate disposal and absorption in diabetes mellitus. Lancet. 1976 Dec 4;2(7997):1213–1216. doi: 10.1016/s0140-6736(76)91142-9. [DOI] [PubMed] [Google Scholar]
  30. Woodside W. F., Heimberg M. Hepatic metabolism of free fatty acids in experimental diabetes. Isr J Med Sci. 1972 Mar;8(3):309–316. [PubMed] [Google Scholar]

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