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. 1982 Dec;70(6):1292–1299. doi: 10.1172/JCI110728

Spontaneous in vitro differentiation of antigen-specific lymphocytes from a patient with immunoglobulin M gammopathy.

J Halper, E A Kabat, E F Osserman, P Tonda, B Pernis
PMCID: PMC370346  PMID: 6983530

Abstract

Recently we have identified two monoclonal immunoglobulin M (IgM) proteins that bind Klebsiella polysaccharides. The lymphocytes of one of these patients (M.A.Y.) were available for study. A substantial proportion of the B lymphocytes isolated from this patient's peripheral blood also bound Klebsiella polysaccharides with a pattern of specificity identical to that of the monoclonal IgM, and reacted with an anti-idiotypic antiserum directed against this IgM. Stripping the surface immunoglobulin from these lymphocytes eliminated this reactivity. Although no plasma cells were detected in the freshly isolated peripheral blood lymphocytes of this patient, plasma cells binding Klebsiella polysaccharide appeared after 7 d of in vitro culture. This occurred regardless of whether the cultures were supplemented with autologous plasma, normal human plasma, or fetal calf serum. Pokeweed mitogen neither stimulated nor inhibited the in vitro differentiation of the monoclonal B lymphocytes into plasma cells. This differentiation was, however, abrogated by F(ab')2 fragments of anti-human IgM and by anti-idiotypic antibodies, as well as by the Klebsiella polysaccharide with which the monoclonal IgM reacted.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbas A. K. Antigen and T lymphocyte mediated suppression of myeloma cells: model systems for regulation of lymphocyte function. Immunol Rev. 1979;48:245–264. doi: 10.1111/j.1600-065x.1979.tb00305.x. [DOI] [PubMed] [Google Scholar]
  2. Bona C. A., Fauci A. S. In vitro idiotypic suppression of chronic lymphocytic leukemia lymphocytes secreting monoclonal immunoglobulin M anti-sheep erythrocyte antibody. J Clin Invest. 1980 Mar;65(3):761–767. doi: 10.1172/JCI109724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bonagura V. R., Kunkel H. G., Pernis B. Cellular localization of rheumatoid factor idiotypes. J Clin Invest. 1982 Jun;69(6):1356–1365. doi: 10.1172/JCI110575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feizi T., Wernet P., Kunkel H. G., Douglas S. D. Lymphocytes forming red cell rosettes in the cold in patients with chronic cold agglutinin disease. Blood. 1973 Nov;42(5):753–762. [PubMed] [Google Scholar]
  5. Finkelman F. D., Lipsky P. E. The role of cell membrane immunoglobulin in primate B lymphocyte differentiation. Immunol Rev. 1979;45:117–139. doi: 10.1111/j.1600-065x.1979.tb00275.x. [DOI] [PubMed] [Google Scholar]
  6. Fu S. M., Chiorazzi N., Kunkel H. G., Halper J. P., Harris S. R. Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes. J Exp Med. 1978 Dec 1;148(6):1570–1578. doi: 10.1084/jem.148.6.1570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halper J., Fu S. M., Wang C. Y., Winchester R., Kunkel H. G. Patterns of expression of human "Ia-like" antigens during the terminal stages of B cell development. J Immunol. 1978 May;120(5):1480–1484. [PubMed] [Google Scholar]
  8. Hammarström L., Smith C. I., Pettersson D., Mellstedt H., Holm G. The protein-A plaque assay: a new system for the detection of cells secreting a given idiotype. J Immunol. 1980 Jan;124(1):140–142. [PubMed] [Google Scholar]
  9. Howard J. G. Cellular events in the induction and loss of tolerance to pneumococcal polysaccharides. Transplant Rev. 1972;8:50–75. doi: 10.1111/j.1600-065x.1972.tb01564.x. [DOI] [PubMed] [Google Scholar]
  10. Kabat E. A., Liao J., Bretting H., Franklin E. C., Geltner D., Frangione B., Koshland M. E., Shyong J., Osserman E. F. Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose. J Exp Med. 1980 Oct 1;152(4):979–995. doi: 10.1084/jem.152.4.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Keightley R. G., Cooper M. D., Lawton A. R. The T cell dependence of B cell differentiation induced by pokeweed mitogen. J Immunol. 1976 Nov;117(5 Pt 1):1538–1544. [PubMed] [Google Scholar]
  12. Lawton A. R., Kincade P. W., Cooper M. D. Sequential expression of germ line genes in development of immunoglobulin class diversity. Fed Proc. 1975 Jan;34(1):33–39. [PubMed] [Google Scholar]
  13. Mudawwar F., Awdeh Z., Ault K., Geha R. S. Regulation of monoclonal immunoglobulin G synthesis by antiidiotypic antibody in a patient with hypogammaglobulinemia. J Clin Invest. 1980 May;65(5):1202–1209. doi: 10.1172/JCI109775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pernis B., Brouet J. C., Seligmann M. IgD and IgM on the membrane of lymphoid cells in macroglobulinemia. Evidence for identity of membrane IgD and IgM antibody activity in a case with anti-IgG receptors. Eur J Immunol. 1974 Nov;4(11):776–778. doi: 10.1002/eji.1830041114. [DOI] [PubMed] [Google Scholar]
  15. Schrader J. W., Nossal G. J. Effector cell blockade. A new mechanism of immune hyporeactivity induced by multivalent antigens. J Exp Med. 1974 Jun 1;139(6):1582–1598. doi: 10.1084/jem.139.6.1582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stevens R. H., Saxon A. Antigen-induced suppression of human in vitro pokeweed mitogen-stimulated antibody production. Cell Immunol. 1980 Sep 15;55(1):85–93. doi: 10.1016/0008-8749(80)90139-2. [DOI] [PubMed] [Google Scholar]
  17. Winchester R. J., Fu S. M., Hoffman T., Kunkel H. G. IgG on lymphocyte surfaces; technical problems and the significance of a third cell population. J Immunol. 1975 Apr;114(4):1210–1212. [PubMed] [Google Scholar]

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