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. 1986 Jun;77(6):1756–1761. doi: 10.1172/JCI112498

Anti-F(ab')2 antibodies in thrombocytopenic patients at risk for acquired immunodeficiency syndrome.

J R Yu, E T Lennette, S Karpatkin
PMCID: PMC370530  PMID: 3011860

Abstract

22 homosexual or narcotic addict patients at risk for acquired immunodeficiency syndrome (AIDS) or with AIDS, were studied for the presence of antiimmunoglobulin antibodies and circulating immune complexes (20 were thrombocytopenic, 6 had AIDS). Circulating immune complex levels were 10-fold higher than levels in normal subjects. IgG anti-F(ab')2 antibodies were noted in homosexual as well as narcotic addict patients. Of 16 homosexual patients, 7 had IgG anti-F(ab')2 antibody of moderate to marked titer with broad reactivity against autologous, homologous, and control F(ab')2 fragments. Three others demonstrated limited reactivity against one or two F(ab')2 fragments. The remaining six patients were negative. Six of six narcotic addict patients had IgG anti-F(ab')2 antibody, five with limited reactivity, one with broad reactivity. In contrast, neither elevated circulating immune complexes nor anti-F(ab')2 antibodies were detectable in six autoimmune thrombocytopenic patients. Anti-F(ab')2 antibody could be affinity purified from serum or circulating immune complexes. Anti-F(ab')2 reactivity correlated with circulating immune complex levels, r = 0.83, P less than 0.01.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRUZZO J. L., CHRISTIAN C. L. The induction of a rheumatoid factor-like substance in rabbits. J Exp Med. 1961 Nov 1;114:791–806. doi: 10.1084/jem.114.5.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. AHO K., WAGER O. Production of anti-antibodies in rabbits. Ann Med Exp Biol Fenn. 1961;39:79–87. [PubMed] [Google Scholar]
  3. Birdsall H. H., Lidsky M. D., Rossen R. D. Anti-Fab' antibodies in rheumatoid arthritis. Measurement of the relative quantities incorporated in soluble immune complexes in sera and supernatants from cultured peripheral blood lymphocytes. Arthritis Rheum. 1983 Dec;26(12):1481–1492. doi: 10.1002/art.1780261210. [DOI] [PubMed] [Google Scholar]
  4. Birdsall H. H., Lidsky M. D., Rossen R. D. Anti-Fab' antibodies in rheumatoid arthritis. Measurement of the relative quantities incorporated in soluble immune complexes in sera and supernatants from cultured peripheral blood lymphocytes. Arthritis Rheum. 1983 Dec;26(12):1481–1492. doi: 10.1002/art.1780261210. [DOI] [PubMed] [Google Scholar]
  5. Birdsall H. H., Rossen R. D. Characterization of anti-Fab' antibodies in human sera: identification of soluble immune complexes that contain hidden anti-KLH and blocking anti-immunoglobulins following immunization with keyhole limpet haemocyanin. Clin Exp Immunol. 1983 Aug;53(2):497–504. [PMC free article] [PubMed] [Google Scholar]
  6. HARBOE M., RAU B., AHO K. PROPERTIES OF VARIOUS ANTI-GAMMA-GLOBULIN FACTORS IN HUMAN SERA. J Exp Med. 1965 Apr 1;121:503–519. doi: 10.1084/jem.121.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heimer R., Wolfe L. D., Abruzzo J. L. IgM and IgG anti-F(ab')2 antibodies in rheumatoid arthritis and systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1298–1306. doi: 10.1002/art.1780251105. [DOI] [PubMed] [Google Scholar]
  8. Karpatkin S. Autoimmune thrombocytopenic purpura. Semin Hematol. 1985 Oct;22(4):260–288. [PubMed] [Google Scholar]
  9. McDougal J. S., Redecha P. B., Inman R. D., Christian C. L. Binding of immunoglobulin G aggregates and immune complexes in human sera to Staphylococci containing protein A. J Clin Invest. 1979 Apr;63(4):627–636. doi: 10.1172/JCI109345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morgan A. C., Jr, Rossen R. D., Twomey J. J. Naturally occurring circulating immune complexes: normal human serum contains idiotype-anti-idiotype complexes dissociable by certain IgG antiglobulins. J Immunol. 1979 May;122(5):1672–1680. [PubMed] [Google Scholar]
  11. Morris L., Distenfeld A., Amorosi E., Karpatkin S. Autoimmune thrombocytopenic purpura in homosexual men. Ann Intern Med. 1982 Jun;96(6 Pt 1):714–717. doi: 10.7326/0003-4819-96-6-714. [DOI] [PubMed] [Google Scholar]
  12. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  13. Nasu H., Chia D. S., Knutson D. W., Barnett E. V. Naturally occurring human antibodies to the F(ab')2 portion of IgG. Clin Exp Immunol. 1980 Nov;42(2):378–386. [PMC free article] [PubMed] [Google Scholar]
  14. Nasu H., Chia D. S., Taniguchi O., Barnett E. V. Characterization of anti-F(ab')2 antibodies in SLE patients. Evidence for cross-reacting auto-anti-idiotypic antibodies. Clin Immunol Immunopathol. 1982 Oct;25(1):80–90. doi: 10.1016/0090-1229(82)90167-2. [DOI] [PubMed] [Google Scholar]
  15. Persselin J. E., Stevens R. H. Anti-Fab antibodies in humans. Predominance of minor immunoglobulin G subclasses in rheumatoid arthritis. J Clin Invest. 1985 Aug;76(2):723–730. doi: 10.1172/JCI112027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ratnoff O. D., Menitove J. E., Aster R. H., Lederman M. M. Coincident classic hemophilia and "idiopathic" thrombocytopenic purpura in patients under treatment with concentrates of antihemophilic factor (factor VIII). N Engl J Med. 1983 Feb 24;308(8):439–442. doi: 10.1056/NEJM198302243080808. [DOI] [PubMed] [Google Scholar]
  17. Savona S., Nardi M. A., Lennette E. T., Karpatkin S. Thrombocytopenic purpura in narcotics addicts. Ann Intern Med. 1985 Jun;102(6):737–741. doi: 10.7326/0003-4819-102-6-737. [DOI] [PubMed] [Google Scholar]
  18. Silvestris F., Bankhurst A. D., Searles R. P., Williams R. C., Jr Studies of anti-F(ab')2 antibodies and possible immunologic control mechanisms in systemic lupus erythematosus. Arthritis Rheum. 1984 Dec;27(12):1387–1396. doi: 10.1002/art.1780271209. [DOI] [PubMed] [Google Scholar]
  19. WILLIAMS R. C., Jr, KUNKEL H. G. Antibodies to rabbit gamma-globulin after immunizing with various preparations of autologous gamma-globulin. Proc Soc Exp Biol Med. 1963 Mar;112:554–561. doi: 10.3181/00379727-112-28104. [DOI] [PubMed] [Google Scholar]
  20. Walsh C. M., Nardi M. A., Karpatkin S. On the mechanism of thrombocytopenic purpura in sexually active homosexual men. N Engl J Med. 1984 Sep 6;311(10):635–639. doi: 10.1056/NEJM198409063111004. [DOI] [PubMed] [Google Scholar]
  21. Walsh C., Krigel R., Lennette E., Karpatkin S. Thrombocytopenia in homosexual patients. Prognosis, response to therapy, and prevalence of antibody to the retrovirus associated with the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Oct;103(4):542–545. doi: 10.7326/0003-4819-103-4-542. [DOI] [PubMed] [Google Scholar]
  22. van Leeuwen E. F., van der Ven J. T., Engelfriet C. P., von dem Borne A. E. Specificity of autoantibodies in autoimmune thrombocytopenia. Blood. 1982 Jan;59(1):23–26. [PubMed] [Google Scholar]

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