Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1981 Apr;67(4):1042–1046. doi: 10.1172/JCI110115

Pyrroline-5-carboxylate reductase in human erythrocytes.

G C Yeh, S C Harris, J M Phang
PMCID: PMC370662  PMID: 6894153

Abstract

Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes. The physiologic function of the enzyme in erythrocytes cannot be related to its function in other tissues, i.e., producing proline for protein synthesis. We examined the kinetic properties of erythrocyte pyrroline-5-carboxylate reductase and compared them to the properties of the enzyme from proliferating cultured human fibroblasts. We found that the kinetic properties and regulation of the erythrocyte enzyme are distinctly different from those for human fibroblast pyrroline-5-carboxylate reductase. These characteristics are consistent with the interpretation that the function of the enzyme in human erythrocytes may be to generate oxidizing potential in the form of NADP+.

Full text

PDF
1042

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams E. Metabolism of proline and of hydroxyproline. Int Rev Connect Tissue Res. 1970;5:1–91. doi: 10.1016/b978-0-12-363705-5.50007-5. [DOI] [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Glatzle D., Körner W. F., Christeller S., Wiss O. Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH2-dependent glutathione reductase from human erythrocytes by FAD in vitro. Investigations on the vitamin B2 status in healthly people and geriatric patients. Int Z Vitaminforsch. 1970;40(2):166–183. [PubMed] [Google Scholar]
  4. Herzfeld A., Mezl V. A., Knox W. E. Enzymes metabolizing delta1-pyrroline-5-carboxylate in rat tissues. Biochem J. 1977 Jul 15;166(1):95–103. doi: 10.1042/bj1660095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Karpatkin S. Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate, and agglutination and contraction on platelet glycolysis. J Clin Invest. 1967 Mar;46(3):409–417. doi: 10.1172/JCI105542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. PEISACH J., STRECKER H. J. The interconversion of glutamic acid and proline. V. The reduction of delta 1-pyrroline-5-carboxylic acid to proline. J Biol Chem. 1962 Jul;237:2255–2260. [PubMed] [Google Scholar]
  8. Phang J. M., Downing S. J., Valle D. A radioisotopic assay for delta1-pyrroline-5-carboxylate reductase. Anal Biochem. 1973 Sep;55(1):266–271. doi: 10.1016/0003-2697(73)90312-6. [DOI] [PubMed] [Google Scholar]
  9. SMITH M. E., GREENBERG D. M. Preparation and properties of partially purified glutamic semialdehyde reductase. J Biol Chem. 1957 May;226(1):317–327. [PubMed] [Google Scholar]
  10. STRECKER H. J. The interconversion of glutamic acid and proline. I. The formation of delta1-pyrroline-5-carboxylic acid from glutamic acid in Escherichia coli. J Biol Chem. 1957 Apr;225(2):825–834. [PubMed] [Google Scholar]
  11. Smith R. J., Downing S. J., Phang J. M. Enzymatic synthesis and purification of L-pyrroline-5-carboxylic acid. Anal Biochem. 1977 Sep;82(1):170–176. doi: 10.1016/0003-2697(77)90145-2. [DOI] [PubMed] [Google Scholar]
  12. Valle D., Blaese R. M., Phang J. M. Increased sensitivity of lymphocyte delta1-pyrroline-5-carboxylate reductase to inhibition by proline with transformation. Nature. 1975 Jan 17;253(5488):214–216. doi: 10.1038/253214a0. [DOI] [PubMed] [Google Scholar]
  13. Valle D., Downing S. J., Phang J. M. Proline inhibition of pyrroline-5-carboxylate reductase: differences in enzymes obtained from animal and tissue culture sources. Biochem Biophys Res Commun. 1973 Oct 15;54(4):1418–1424. doi: 10.1016/0006-291x(73)91144-3. [DOI] [PubMed] [Google Scholar]
  14. Yeh G. C., Phang J. M. The function of pyrroline-5-carboxylate reductase in human erythrocytes. Biochem Biophys Res Commun. 1980 May 30;94(2):450–457. doi: 10.1016/0006-291x(80)91252-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES