Abstract
Bovine aortic endothelial and smooth muscle cells in culture were incubated with arachidonic acid or prostaglandin H2. The amount of prostacyclin nd thromboxane A2 synthesized ws then determined by specific radioimmunoassay for 6-keto-prostaglandin F1 alpha and thromboxane B2. Although smooth muscle cells produced only 6-keto-prostaglandin F1 alpha and thromboxane B2 in a ratio of 5:1 to 10:1. The same ratio of these metabolites of arachidonic acid ws also found when prostaglandin production from endogenous arachidonic acid was stimulated in endothelial cells by the ionophore A23187. Cyclooxygenase inhibitors inhibited the production of both metabolites equally, whereas thromboxane synthetase inhibitors selectively inhibited the production of thromboxane B2. Cells in culture were also incubated with [1-14C]arachidonic acid and the extracted products were identified by two-dimensional thin-layer chromatography. 6-Keto-prostaglandin F1 alpha was the only metabolite produced by smooth muscle cells, but endothelial cells synthesized 6-keto prostaglandin F1 alpha, thromboxane B2, prostaglandin E2, and prostaglandin F2 alpha.
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Selected References
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- Ali A. E., Barrett J. C., Eling T. E. Prostaglandin and thromboxane production by fibroblasts and vascular endothelial cells. Prostaglandins. 1980 Oct;20(4):667–688. doi: 10.1016/0090-6980(80)90107-0. [DOI] [PubMed] [Google Scholar]
- Hamberg M., Samuelsson B. Prostaglandins in human seminal plasma. Prostaglandins and related factors 46. J Biol Chem. 1966 Jan 25;241(2):257–263. [PubMed] [Google Scholar]
- Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lefer A. M., Smith E. F., 3rd, Araki H., Smith J. B., Aharony D., Claremon D. A., Magolda R. L., Nicolaou K. C. Dissociation of vasoconstrictor and platelet aggregatory activities of thromboxane by carbocyclic thromboxane A2, a stable analog of thromboxane A2. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1706–1710. doi: 10.1073/pnas.77.3.1706. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine L., Alam I. Arachidonic acid metabolism by cells in culture: analyses of culture fluids for cyclooxygenase products by radioimmunoassay before and after separation by high pressure liquid chromatography. Prostaglandins Med. 1979 Nov;3(5):295–304. doi: 10.1016/0161-4630(79)90071-5. [DOI] [PubMed] [Google Scholar]
- Macarak E. J., Howard B. V., Kefalides N. A. Properties of calf endothelial cells in culture. Lab Invest. 1977 Jan;36(1):62–67. [PubMed] [Google Scholar]
- Maurer P., Moskowitz M. A., Levine L., Melamed E. The synthesis of prostaglandins by bovine cerebral microvessels. Prostaglandins Med. 1980 Mar;4(3):153–161. doi: 10.1016/0161-4630(80)90077-4. [DOI] [PubMed] [Google Scholar]
- Moncada S., Gryglewski R., Bunting S., Vane J. R. An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature. 1976 Oct 21;263(5579):663–665. doi: 10.1038/263663a0. [DOI] [PubMed] [Google Scholar]
- Nugteren D. H., Hazelhof E. Isolation and properties of intermediates in prostaglandin biosynthesis. Biochim Biophys Acta. 1973 Dec 20;326(3):448–461. doi: 10.1016/0005-2760(73)90145-8. [DOI] [PubMed] [Google Scholar]
- Salzman P. M., Salmon J. A., Moncada S. Prostacyclin and thromboxane A2 synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther. 1980 Oct;215(1):240–247. [PubMed] [Google Scholar]
- Tuvemo T., Strandberg K., Hamberg M., Samuelsson B. Formation and action of prostaglandin endoperoxides in the isolated human umbilical artery. Acta Physiol Scand. 1976 Feb;96(2):145–149. doi: 10.1111/j.1748-1716.1976.tb10183.x. [DOI] [PubMed] [Google Scholar]