Skip to main content
. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Oncogene. 2012 Jan 16;31(44):4709–4717. doi: 10.1038/onc.2011.622

Figure 4. Involvement of Mediator complex subunits in Rb/E2F-mediated growth arrest.

Figure 4

(A) A highly interconnected node of candidate genes identified in the screen was revealed by Ingenuity Pathways Analysis. One identified network is shown. Shading indicates gene products identifed in the shRNA screen. MED13L, MED27, ZCCHC10, DDX52, TARDBP, and DNAJA1 were connected by reciprocal protein-protein interactions. (B) T98G-EcoR cells expressing shRNA sequences targeting luciferase (shLuc) or shRNAs targeting MED27, MED13L, DNAJA1, ZCCHC10, TARDBP, or DDX52 were generated by retroviral infection and selection. Polyclonal populations were subsequently infected with retrovirus producing PSM-Rb, selected in hygromycin for 19 days, fixed, and stained with crystal violet. The number of colonies formed was counted and graphed relative to the control (shLuc) plate. The mean ± S.D. is shown for two independent experiments. * indicates p<0.05. ** indicates p<0.01. (C) Representative images collected from dishes of cells as described in (B).