Fig. 1.

Genomic organization of the VCBP2/5 gene cluster. A In Brafl1, the reference genome animal, two VCBP5 paralogs are separated by a VCBP2 gene, which lies within a larger chromosomal region encoding other VCBP genes (Dishaw et al. 2008). Chromosomal distances in (A) are to scale. B Copy number variants (CNVs) observed in different VCBP2/5 haplotypes are depicted in grey shading. C VCBP2 and VCBP5 genes consist of seven exons (EX1-7) that encode two V-type Ig domains and one chitin-binding domain as illustrated for the 5b gene locus. A region of elevated, localized, polymorphism in exon 2 (EX2, hotspot motif) is shaded. Nucleotide sequence length in the reference BAC contig 63n5-43b24 is indicated below introns and exons. Arrows below EX1 and EX3 depict the location of priming sites that were used for PCR genotyping of 5a, 2b, and 5b genes