Abstract
We report the complete genomic sequences of an avian orthoreovirus, strain GuangxiR1, isolated from a chicken flock in Guangxi Province, southern China, in 2000. Phylogenetic analyses suggest that the strain is closely related to the S1133 strain, which is associated with tenosynovitis, but is far different from strain AVS-B, which is associated with runting-stunting syndrome in broilers.
GENOME ANNOUNCEMENT
Avian orthoreovirus (ARV) is the etiological agent of viral arthritis, runting-stunting syndrome, and tenosynovitis in chickens, causing significant economic losses in the poultry industry (1, 2). Avian orthoreovirus belongs to the genus Orthoreovirus, family Reoviridae (3). The genome of 10 RNA segments was divided into three size classes, designated large (L1, L2, L3), medium (M1, M2, M3), and small (S1, S2, S3, and S4) (4). These segments encode 10 viral structural proteins (λA, λB, λC, μA, μB, μBC, μBN, σC, σA, and σB) and five nonstructural proteins (μNSC, μNS, σNS, P10, and P17) (5, 6, 7). The genetic characterization of the complete genomic sequence is limited to a few strains (8).
The avian orthoreovirus strain GuangxiR1, which caused paralysis and hock joints in commercial broilers, was isolated from Guangxi, southern China, in 2000 (9, 10). The nucleotide sequences of GuangxiR1 were amplified through reverse transcription PCR (RT-PCR). The amplified products were purified using a DNA purification kit (TaKaRa Biotechnology) and cloned into pMD18-T vector (TaKaRa, Dalian, China). The PCR product DNA sequencing was performed by two commercial DNA sequence service companies (Invitrogen, Biotechnology Co., Ltd. and Shenzhen Huada Genomics Co., Ltd.). Nucleotide sequence analysis and alignment were done using the MEGA v. 4.1 software package (MEGA Software).
The complete genome of GuangxiR1 is 23,494 bp. The full lengths of the L1, L2, L3, M1, M2, M3, S1, S2, S3, and S4 segments are 3,959, 3,830, 3,907, 2,283, 2,158, 1,996, 1,643, 1,324, 1,202, and 1,192 nucleotides, respectively. The deduced lengths of the structural and nonstructural proteins λa, λB, λC, μA, μB, μNS, σC, σA, σB, and σNS are 1,293, 1,259, 1,285, 732, 676, 635, 326, 416, 367, and 367 amino acids, respectively. The σC protein, involved in the induction of apoptosis, is coded by the S1 segment. Amino acid comparative analyses indicated that σC shares the highest sequence homology (98.2%) with that from the S1133 strain, associated with tenosynovitis (GenBank accession no. AF330703), but only 48.5% with that from the AVS-B strain, associated with runting-stunting syndrome in broilers (GenBank accession no. FR694197). These data are useful for analyses of the epidemiology and evolutionary characteristics of avian orthoreovirus GuangxiR1.
Nucleotide sequence accession numbers.
The complete genome sequences of GuangxiR1 are available in GenBank. The accession no. KC183743 to KC183752 correspond to L1, L2, L3, M1, M2, M3, S1, S2, S3, and S4, respectively.
ACKNOWLEDGMENTS
This work was supported by the National Natural Science Foundation of China (no. 31160512), the Guangxi Science and Technology Bureau (no. 1222003-2-4), and the Guangxi Government Senior Scientist Foundation (no. 2011B020).
Footnotes
Citation Teng L, Xie Z, Xie L, Liu J, Pang Y, Deng X, Xie Z, Fan Q, Luo S. 2013. Complete genome sequences of an avian orthoreovirus isolated from Guangxi, China. Genome Announc. 1(4):e00495-13. doi:10.1128/genomeA.00495-13.
REFERENCES
- 1. Glass SE, Naqi SA, Hall CF, Kerr KM. 1973. Isolation and characterization of a virus associated with arthritis of chickens. Avian Dis. 17:415–424 [PubMed] [Google Scholar]
- 2. Hieronymus DR, Villegas P, Kleven SH. 1983. Identification and serological differentiation of several reovirus strains isolated from chickens with suspected malabsorption syndrome. Avian Dis. 27:246–254 [PubMed] [Google Scholar]
- 3. Spandidos DA, Graham AF. 1976. Physical and chemical characterization of an avian reovirus. J. Virol. 19:968–976 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. Varela R, Benavente J. 1994. Protein coding assignment of avian reovirus strain S1133. J. Virol. 68:6775–6777 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. Bodelón G, Labrada L, Martínez-Costas J, Benavente J. 2001. The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells. Virology 290:181–191 [DOI] [PubMed] [Google Scholar]
- 6. Shih WL, Hsu HW, Liao MH, Lee LH, Liu HJ. 2004. Avian reovirus sigmaC protein induces apoptosis in cultured cells. Virology 321:65–74 [DOI] [PubMed] [Google Scholar]
- 7. Benavente J, Martínez-Costas J. 2007. Avian reovirus: structure and biology. Virus Res. 123:105–119 [DOI] [PubMed] [Google Scholar]
- 8. Bányai K, Dandár E, Dorsey KM, Mató T, Palya V. 2011. The genomic constellation of a novel avian orthoreovirus strain associated with runting-stunting syndrome in broilers. Virus Genes 42:82–89 [DOI] [PubMed] [Google Scholar]
- 9. Liao M, Xie Z, Liu J, Pang Y, Deng X, Xie Z, Tang X. 2002. Isolation and identification of avian reovirus. China Poult. 24:12–14 [Google Scholar]
- 10. Xie Z, Peng Y, Luo S, Wang Y, Liu J, Pang Y, Deng X, Xie Z, Xie L, Fan Q, Teng L, Wang X. 2012. Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus. Avian Pathol. 41:311–316 [DOI] [PubMed] [Google Scholar]