Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1981 Dec;68(6):1558–1565. doi: 10.1172/JCI110410

Isolation and partial characterization of Fc gamma-binding proteins of human leukocytes.

A Kulczycki Jr, L Solanki, L Cohen
PMCID: PMC370960  PMID: 7033289

Abstract

We isolated and partially characterized three Fc-binding macromolecules from human leukocytes. Mononuclear cells from normal individuals and from five patients with chronic lymphocytic leukemia and neutrophils from normal donors were surface radiolabeled by using 125I and lactoperoxidase. After detergent solubilization of the cells, Fc gamma-binding macromolecules were purified by repetitive affinity chromatography under a variety of conditions and analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Three radiolabeled macromolecules were isolated that retained specific ability to bind to Fc fragments. A 52,000-64,000-mol wt macromolecule was isolated from normal mononuclear and polymorphonuclear cells. A 43,000-mol wt band was characteristic of mononuclear cells, particularly from patients with chronic lymphocytic leukemia. A 33,000-mol wt molecule could be obtained from normal leukocytes under conditions that suggest it might be a proteolytic fragment.

Full text

PDF
1558

Images in this article

1560Image
on p.1560
1561Image
on p.1561
1562Image
on p.1562
1562Image
on p.1562
1563Image
on p.1563
1563Image
on p.1563

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcon-Segovia D., Ruiz-Arguelles A., Fishbein E. Antibody to nuclear ribonucleoprotein penetrates live human mononuclear cells through Fc receptors. Nature. 1978 Jan 5;271(5640):67–69. doi: 10.1038/271067a0. [DOI] [PubMed] [Google Scholar]
  2. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benavides I., Catovsky D. Myeloperoxidase cytochemistry using 2, 7-fluorenediamine. J Clin Pathol. 1978 Nov;31(11):1114–1116. doi: 10.1136/jcp.31.11.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bourgois A., Abney E. R., Parkhouse R. M. Structure of mouse Fc receptor. Eur J Immunol. 1977 Oct;7(10):691–695. doi: 10.1002/eji.1830071008. [DOI] [PubMed] [Google Scholar]
  6. Conrad D. H., Froese A. Characterization of the target cell receptor for IgE. II. Polyacrylamide gel analysis of the surface IgE receptor from normal rat mast cells and from rat basophilic leukemia cells. J Immunol. 1976 Feb;116(2):319–326. [PubMed] [Google Scholar]
  7. Conrad D. H., Froese A. Characterization of the target cell receptor for IgE. III. properties of the receptor isolated from rat basophilic leukemia cells by affinity chromatography. J Immunol. 1978 Feb;120(2):429–437. [PubMed] [Google Scholar]
  8. Cooper S. M., Sambray Y. Characterization of the Fc receptors of the murine leukemia L1210. J Supramol Struct. 1977;6(4):591–597. doi: 10.1002/jss.400060412. [DOI] [PubMed] [Google Scholar]
  9. Cunningham-Rundles C., Lawless D., Gupta S., Galanos C., Good R. A. Isolation and partial chemical characterization of the IgG Fc receptor of human T lymphocytes and production of an antiserum. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3645–3648. doi: 10.1073/pnas.77.6.3645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cunningham-Rundles C., Siegal F. P., Good R. A. Isolation and characterization of a human mononuclear cell Fc receptor. Immunochemistry. 1978 Jun;15(6):365–370. doi: 10.1016/0161-5890(78)90132-3. [DOI] [PubMed] [Google Scholar]
  11. D'Urso-Coward M., Cone R. E. Membrane proteins of the P388D1 macrophage cell line: isolation of membrane polypeptides that bind to the Fc portion of aggregated IgG. J Immunol. 1978 Nov;121(5):1973–1980. [PubMed] [Google Scholar]
  12. Dickler H. B. Lymphocyte receptors for immunoglobulin. Adv Immunol. 1976;24:167–214. doi: 10.1016/s0065-2776(08)60330-2. [DOI] [PubMed] [Google Scholar]
  13. Eisen S. A., Wedner H. J., Parker C. W. Isolation of pure human peripheral blood T-lymphocytes using nylon wool columns. Immunol Commun. 1972;1(6):571–577. doi: 10.3109/08820137209022965. [DOI] [PubMed] [Google Scholar]
  14. Faguet G. B. Lymphocyte purification: an improved method. Qualitative and quantitative evaluation. Biomedicine. 1974 Apr 10;21(4):153–157. [PubMed] [Google Scholar]
  15. Fechheimer M., Daiss J. L., Cebra J. J. Interaction of immunoglobulin with actin. Mol Immunol. 1979 Nov;16(11):881–888. doi: 10.1016/0161-5890(79)90086-5. [DOI] [PubMed] [Google Scholar]
  16. Hempstead B. L., Parker C. W., Kulczycki A., Jr Characterization of the IgE receptor isolated from human basophils. J Immunol. 1979 Nov;123(5):2283–2291. [PubMed] [Google Scholar]
  17. Huber H., Fudenberg H. H. Receptor sites of human monocytes for IgG. Int Arch Allergy Appl Immunol. 1968;34(1):18–31. doi: 10.1159/000230091. [DOI] [PubMed] [Google Scholar]
  18. Kahn-Perles B., Sire J., Boned A., Bourgois A. Putative conformation of mouse Fc gamma-receptor. J Immunol. 1980 Sep;125(3):1360–1366. [PubMed] [Google Scholar]
  19. Kanellopoulos J., Rossi G., Metzger H. Preparative isolation of the cell receptor for immunoglobulin E. J Biol Chem. 1979 Aug 25;254(16):7691–7697. [PubMed] [Google Scholar]
  20. Kulczycki A., Jr, Hempstead B. L., Hofmann S. L., Wood E. W., Parker C. W. The cell surface receptor for immunoglobulin E. II. Properties of the purified biologically active receptor. J Biol Chem. 1979 May 10;254(9):3194–3200. [PubMed] [Google Scholar]
  21. Kulczycki A., Jr, Krause V., Killion C. C., Atkinson J. P. Improved cell surface radioiodination of macrophages. J Immunol Methods. 1980;37(2):133–138. doi: 10.1016/0022-1759(80)90198-2. [DOI] [PubMed] [Google Scholar]
  22. Kulczycki A., Jr, Krause V., Killion C. C., Atkinson J. P. Purification of Fc gamma receptor from rabbit alveolar macrophages that retains ligand-binding activity. J Immunol. 1980 Jun;124(6):2772–2779. [PubMed] [Google Scholar]
  23. Kulczycki A., Jr, McNearney T. A., Parker C. W. The rat basophilic leukemia cell receptor for IgE. I. Characterization as a glycoprotein. J Immunol. 1976 Aug;117(2):661–665. [PubMed] [Google Scholar]
  24. Kulczycki A., Jr, Parker C. W. The cell surface receptor for immunoglobulin E. I. The use of repetitive affinity chromatography for the purification of a mammalian receptor. J Biol Chem. 1979 May 10;254(9):3187–3193. [PubMed] [Google Scholar]
  25. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  26. Lane B. C., Kan-Mitchell J., Mitchell M. S., Cooper S. M. Structural evidence for distinct IgG subclass-specific Fc receptors on mouse peritoneal macrophages. J Exp Med. 1980 Nov 1;152(5):1147–1161. doi: 10.1084/jem.152.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Lazarides E., Lindberg U. Actin is the naturally occurring inhibitor of deoxyribonuclease I. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4742–4746. doi: 10.1073/pnas.71.12.4742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Loube S. R., Dorrington K. J. Isolation of biosynthetically-labeled Fc-binding proteins from detergent lysates and spent culture fluid of a macrophage-like cell line (P388D1). J Immunol. 1980 Sep;125(3):970–975. [PubMed] [Google Scholar]
  29. Loube S. R., McNabb T. C., Dorrington K. J. Isolation of an Fcgamma-binding protein from the cell membrane of a macrophage-like cell line (P388D1) after detergent solubilization. J Immunol. 1978 Mar;120(3):709–715. [PubMed] [Google Scholar]
  30. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  31. Mantovani B., Rabinovitch M., Nussenzweig V. Phagocytosis of immune complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG). J Exp Med. 1972 Apr 1;135(4):780–792. doi: 10.1084/jem.135.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Mellman I. S., Unkeless J. C. Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody. J Exp Med. 1980 Oct 1;152(4):1048–1069. doi: 10.1084/jem.152.4.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Messner R. P., Jelinek J. Receptors for human gamma G globulin on human neutrophils. J Clin Invest. 1970 Dec;49(12):2165–2171. doi: 10.1172/JCI106435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Moosic J. P., Nilson A., Hämmerling G. J., McKean D. J. Biochemical characterization of Ia antigens. I. Characterization of the 31K polypeptide associated with I-A subregion Ia antigens. J Immunol. 1980 Oct;125(4):1463–1469. [PubMed] [Google Scholar]
  35. Owen M. J., Auger J., Barber B. H., Edwards A. J., Walsh F. S., Crumpton M. J. Actin may be present on the lymphocyte surface. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4484–4488. doi: 10.1073/pnas.75.9.4484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Rask L., Klarkeskog L., Ostberg L., Peterson P. A. Isolation and properties of a murine spleen cell Fc receptor. Nature. 1975 Sep 18;257(5523):231–233. doi: 10.1038/257231a0. [DOI] [PubMed] [Google Scholar]
  37. Sire J., Kahn-Perles B., Collé A., Bourgois A. Biochemical characterization of an Fc receptor of rabbit lymphocytes. Eur J Immunol. 1980 Feb;10(2):116–121. doi: 10.1002/eji.1830100209. [DOI] [PubMed] [Google Scholar]
  38. Suzuki T., Taki T., Hachimine K., Sadasivan R. Biochemical properties of biologically active Fc gamma receptors of human B lymphocytes. Mol Immunol. 1981 Jan;18(1):55–65. doi: 10.1016/0161-5890(81)90048-1. [DOI] [PubMed] [Google Scholar]
  39. Takacs B. J. Characterization of functional Fc receptor material from human lymphoblastoid cell lines--I. Large scale purification and biochemical analysis. Mol Immunol. 1980 Oct;17(10):1293–1314. doi: 10.1016/0161-5890(80)90026-7. [DOI] [PubMed] [Google Scholar]
  40. Thoenes J., Stein H. Properties of an Fc gamma-binding protein isolated from human leukemic B cells. J Exp Med. 1979 Nov 1;150(5):1049–1066. doi: 10.1084/jem.150.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES