Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1981 Dec;68(6):1577–1585. doi: 10.1172/JCI110412

Characterization of the suppressor cell activity in human cord blood lymphocytes.

M A Rodriguez, A D Bankhurst, J L Ceuppens, R C Williams Jr
PMCID: PMC370962  PMID: 6172447

Abstract

Newborn mononuclear cells are known to have increased suppressor activity when compared with adult cells. However, the precise phenotypic description of the suppressor cell subpopulation has not yet been reported. This study was designed to examine the surface markers on human cord blood cells as defined by monoclonal antibodies and to characterize cord blood mononuclear cells that suppress in vitro pokeweed mitogen-driven immunoglobulin production. The pattern of fluorescence with the 9,6-monoclonal antibody suggested either a decreased density or a partial blocking of E-receptors on cord blood lymphocytes. Otherwise, human cord and adult cells had similar proportions of T cell subpopulations. Different subsets of newborn cells isolated by a monoclonal antibody rosetting technique were tested for suppressor activity. Cord blood lymphocytes recognized by the OKT8 monoclonal antibody constituted the major functional suppressor cell subpopulation. These cells were present in both E-rosetting and non-E-rosetting populations. Removal of OKT8+ mononuclear cord blood cells abrogated most of the suppressor activity. Thus the major suppressor cell present in cord blood is an OKT8 positive lymphocyte.

Full text

PDF
1577

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breard J., Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol. 1980 Apr;124(4):1943–1948. [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Durandy A., Fischer A., Griscelli C. Active suppression of B lymphocyte maturation by two different newborn T lymphocyte subsets. J Immunol. 1979 Dec;123(6):2644–2650. [PubMed] [Google Scholar]
  4. Gmelig-Meyling F., Waldmann T. A. Human B cell activation in vitro: augmentation and suppression by monocytes of the immunoglobulin production induced by various B cell stimulants. J Immunol. 1981 Feb;126(2):529–537. [PubMed] [Google Scholar]
  5. Hayward A. R., Kurnick J. Newborn T cell suppression: early appearance, maintenance in culture, and lack of growth factor suppression. J Immunol. 1981 Jan;126(1):50–53. [PubMed] [Google Scholar]
  6. Hayward A. R., Lawton A. R. Induction of plasma cell differentiation of human fetal lymphocytes: evidence for functional immaturity of T and B cells. J Immunol. 1977 Oct;119(4):1213–1217. [PubMed] [Google Scholar]
  7. Hayward A. R., Lydyard P. M. Suppression of B lymphocyte differentiation by newborn T lymphocytes with an Fc receptor for IgM. Clin Exp Immunol. 1978 Dec;34(3):374–378. [PMC free article] [PubMed] [Google Scholar]
  8. Janossy G., Tidman N., Papageorgiou E. S., Kung P. C., Goldstein G. Distribution of t lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies. J Immunol. 1981 Apr;126(4):1608–1613. [PubMed] [Google Scholar]
  9. Jerrells T. R., Dean J. H., Richardson G. L., Herberman R. B. Depletion of monocytes from human peripheral blood mononuclear leukocytes: comparison of the sephadex G-10 column method with other commonly used techniques. J Immunol Methods. 1980;32(1):11–29. doi: 10.1016/0022-1759(80)90113-1. [DOI] [PubMed] [Google Scholar]
  10. Kamoun M., Martin P. J., Hansen J. A., Brown M. A., Siadak A. W., Nowinski R. C. Identification of a human T lymphocyte surface protein associated with the E-rosette receptor. J Exp Med. 1981 Jan 1;153(1):207–212. doi: 10.1084/jem.153.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kasakura S. A factor in maternal plasma during pregnancy that suppresses the reactivity of mixed leukocyte cultures. J Immunol. 1971 Nov;107(5):1296–1301. [PubMed] [Google Scholar]
  12. Lester E. P., Miller J. B., Yachnin S. Human alpha-fetoprotein as a modulator of human lymphocyte transformation: correlation of biological potency with electrophoretic variants. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4645–4648. doi: 10.1073/pnas.73.12.4645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Milgrom H., Shore S. L. Assessment of monocyte function in the normal newborn infant by antibody-dependent cellular cytotoxicity. J Pediatr. 1977 Oct;91(4):612–614. doi: 10.1016/s0022-3476(77)80515-5. [DOI] [PubMed] [Google Scholar]
  14. Miyawaki T., Moriya N., Nagaoki T., Kubo M., Yokoi T., Taniguchi N. Mode of action of humoral suppressor factor derived from pokeweed mitogen-stimulated cord T cells of adult B cell differentiation. J Immunol. 1981 Jan;126(1):282–285. [PubMed] [Google Scholar]
  15. Miyawaki T., Seki H., Kubo M., Taniguchi N. Suppressor activity of T lymphocytes from infants assessed by co-culture with unfractionated adult lymphocytes in the pokeweed mitogen system. J Immunol. 1979 Sep;123(3):1092–1096. [PubMed] [Google Scholar]
  16. Morito T., Bankhurst A. D., Williams R. C., Jr Studies of human cord blood and adult lymphocyte interactions with in vitro immunoglobulin production. J Clin Invest. 1979 Oct;64(4):990–995. doi: 10.1172/JCI109565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moriya N., Nagaoki T., Okuda N., Taniguchi N. Suppression of adult B cell differentiation in pokeweed mitogen-stimulated cultures by Fc(IgG) receptor-negative T cells from cord blood. J Immunol. 1979 Oct;123(4):1795–1798. [PubMed] [Google Scholar]
  18. Mosier D. E., Mathieson B. J., Campbell P. S. Ly phenotype and mechanism of action of mouse neonatal suppressor T cells. J Exp Med. 1977 Jul 1;146(1):59–73. doi: 10.1084/jem.146.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagaoki T., Moriya N., Miyawaki T., Seki H., Kubo M., Yokoi T., Okuda N., Taniguchi N. Suppression of B cell differentiation by dialyzable humoral factors derived from pokeweed mitogen-stimulated cord T cells. J Immunol. 1980 Oct;125(4):1563–1568. [PubMed] [Google Scholar]
  20. Olding L. B., Murgita R. A., Wigzell H. Mitogen-stimulated lymphoid cells from human newborns suppress the proliferation of maternal lymphocytes actoss a cell-impermeable membrane. J Immunol. 1977 Sep;119(3):1109–1114. [PubMed] [Google Scholar]
  21. Olding L. B., Oldstone B. A. Thymus-derived peripheral lymphocytes from human newborns inhibit division of their mothers' lymphocytes. J Immunol. 1976 Mar;116(3):682–686. [PubMed] [Google Scholar]
  22. Oldstone M. B., Tishon A., Moretta L. Active thymus derived suppressor lymphocytes in human cord blood. Nature. 1977 Sep 22;269(5626):333–335. doi: 10.1038/269333a0. [DOI] [PubMed] [Google Scholar]
  23. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol. 1980 Mar;124(3):1301–1307. [PubMed] [Google Scholar]
  24. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol. 1979 Dec;123(6):2894–2896. [PubMed] [Google Scholar]
  25. Reinherz E. L., Moretta L., Roper M., Breard J. M., Mingari M. C., Cooper M. D., Schlossman S. F. Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med. 1980 Apr 1;151(4):969–974. doi: 10.1084/jem.151.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Reinherz E. L., Morimoto C., Penta A. C., Schlossman S. F. Regulation of B cell immunoglobulin secretion by functional subsets of T lymphocytes in man. Eur J Immunol. 1980 Jul;10(7):570–572. doi: 10.1002/eji.1830100715. [DOI] [PubMed] [Google Scholar]
  27. Reinherz E. L., Schlossman S. F. Current concepts in immunology: Regulation of the immune response--inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med. 1980 Aug 14;303(7):370–373. doi: 10.1056/NEJM198008143030704. [DOI] [PubMed] [Google Scholar]
  28. Rocklin R. E., Kitzmiller J. L., Carpenter C. B., Garovoy M. R., David J. R. Maternal-fetal relation. Absence of an immunologic blocking factor from the serum of women with chronic abortions. N Engl J Med. 1976 Nov 25;295(22):1209–1213. doi: 10.1056/NEJM197611252952201. [DOI] [PubMed] [Google Scholar]
  29. Stimson W. H. Studies on the immunosuppressive properties of a pregnancy-associated alpha-macroglobulin. Clin Exp Immunol. 1976 Aug;25(2):199–206. [PMC free article] [PubMed] [Google Scholar]
  30. Stocker J. W., Garotta G., Hausmann B., Trucco M., Ceppellini R. Separation of human cells bearing HLA-DR antigens using a monoclonal antibody rosetting method. Tissue Antigens. 1979 Mar;13(3):212–222. doi: 10.1111/j.1399-0039.1979.tb00786.x. [DOI] [PubMed] [Google Scholar]
  31. Thomas Y., Sosman J., Irigoyen O., Friedman S. M., Kung P. C., Goldstein G., Chess L. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 1980 Dec;125(6):2402–2408. [PubMed] [Google Scholar]
  32. Tosato G., Magrath I. T., Koski I. R., Dooley N. J., Blaese R. M. B cell differentiation and immunoregulatory T cell function in human cord blood lymphocytes. J Clin Invest. 1980 Aug;66(2):383–388. doi: 10.1172/JCI109867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Ugolini V., Nunez G., Smith R. G., Stastny P., Capra J. D. Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6764–6768. doi: 10.1073/pnas.77.11.6764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Witemeyer S., Bankhurst A. D., Williams R. C., Jr A population of human cord blood lymphocytes which generates Fc receptors in vitro. Cell Immunol. 1977 Apr;30(1):54–65. doi: 10.1016/0008-8749(77)90047-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES