Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1981 Dec;68(6):1586–1591. doi: 10.1172/JCI110413

Release of a slow-reacting substance from rabbit platelets.

J M Mencia-Huerta, L Hadji, J Benveniste
PMCID: PMC370963  PMID: 6119324

Abstract

Washed rabbit platelets stimulated with platelet-activating factor, thrombin, or arachidonic acid, released a slow-reacting substance (SRS), whereas platelets aggregated by adenosine diphosphate did not. Production of platelet-derived SRS was neither affected by indomethacin nor aspirin but was reduced by large doses of eicosatetraynoic acid, an inhibitor of the cyclo-oxygenase and lipoxygenase. L-cysteine enhanced markedly the release of SRS from platelets. This SRS activity, which was antagonized by FPL 55712 and inactivated by arylsulfatase, followed the same elution pattern on Amberlite, silicic acid, and reverse phase high-pressure liquid chromatography columns as that described for the SRS from other origins. SRS activity released from platelets preincubated with [14C]arachidonic acid exhibited the same retention time as radioactivity in reverse phase high-pressure liquid chromatography. The release of a SRS from platelets is consistent with their implication in the pathogenesis of asthma and other lung diseases.

Full text

PDF
1586

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ardlie N. G., Packham M. A., Mustard J. F. Adenosine diphosphate-induced platelet aggregation in suspensions of washed rabbit platelets. Br J Haematol. 1970 Jul;19(1):7–17. doi: 10.1111/j.1365-2141.1970.tb01596.x. [DOI] [PubMed] [Google Scholar]
  2. Arnoux B., Duval D., Benveniste J. Release of platelet-activating factor (PAF-acether) from alveolar macrophages by the calcium ionophore A23187 and phagocytosis. Eur J Clin Invest. 1980 Dec;10(6):437–441. doi: 10.1111/j.1365-2362.1980.tb02082.x. [DOI] [PubMed] [Google Scholar]
  3. Augstein J., Farmer J. B., Lee T. B., Sheard P., Tattersall M. L. Selective inhibitor of slow reacting substance of anaphylaxis. Nat New Biol. 1973 Oct 17;245(146):215–217. doi: 10.1038/newbio245215a0. [DOI] [PubMed] [Google Scholar]
  4. Bach M. K., Brashler J. R., Gorman R. R. On the structure of slow reacting substance of anaphylaxis: evidence of biosynthesis from arachidonic acid. Prostaglandins. 1977 Jul;14(1):21–38. doi: 10.1016/0090-6980(77)90154-x. [DOI] [PubMed] [Google Scholar]
  5. Bach M. K., Brashler J. R. In vivo and in vitro production of a slow reacting substance in the rat upon treatment with calcium ionophores. J Immunol. 1974 Dec;113(6):2040–2044. [PubMed] [Google Scholar]
  6. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Benveniste J., Le Couedic J. P., Polonsky J., Tence M. Structural analysis of purified platelet-activating factor by lipases. Nature. 1977 Sep 8;269(5624):170–171. doi: 10.1038/269170a0. [DOI] [PubMed] [Google Scholar]
  8. Benveniste J. Platelet-activating factor, a new mediator of anaphylaxis and immune complex deposition from rabbit and human basophils. Nature. 1974 Jun 7;249(457):581–582. doi: 10.1038/249581a0. [DOI] [PubMed] [Google Scholar]
  9. Benveniste J., Tencé M., Varenne P., Bidault J., Boullet C., Polonsky J. Semi-synthèse et structure proposée du facteur activant les plaquettes (P.A.F.): PAF-acether, un alkyl ether analogue de la lysophosphatidylcholine. C R Seances Acad Sci D. 1979 Nov 26;289(14):1037–1040. [PubMed] [Google Scholar]
  10. Blackwell G. J., Burka J. F., Flower R. J., Torkington P. On the preparation of highly purified slow reacting substance of anaphylaxis (SRS-A) from biological extracts. Br J Pharmacol. 1980 Jan;68(1):33–46. doi: 10.1111/j.1476-5381.1980.tb10696.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cazenave J. P., Benveniste J., Mustard J. F. Aggregation of rabbit platelets by platelet-activating factor is independent of the release reaction and the arachidonate pathway and inhibited by membrane-active drugs. Lab Invest. 1979 Sep;41(3):275–285. [PubMed] [Google Scholar]
  12. Conroy M. C., Orange R. P., Lichtenstein L. M. Release of slow reacting substance of anaphylaxis (SRS-A) from human leukocytes by the calcium ionophore A23187. J Immunol. 1976 Jun;116(6):1677–1681. [PubMed] [Google Scholar]
  13. Demopoulos C. A., Pinckard R. N., Hanahan D. J. Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). J Biol Chem. 1979 Oct 10;254(19):9355–9358. [PubMed] [Google Scholar]
  14. Engineer D. M., Niederhauser U., Piper P. J., Sirois P. Release of mediators of anaphylaxis: inhibition of prostaglandin synthesis and the modification of release of slow reacting substance of anaphylaxis and histamine. Br J Pharmacol. 1978 Jan;62(1):61–66. doi: 10.1111/j.1476-5381.1978.tb07006.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hamberg M., Samuelsson B. Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3400–3404. doi: 10.1073/pnas.71.9.3400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hammarström S. Leukotriene C5: a slow reacting substance derived from eicosapentaenoic acid. J Biol Chem. 1980 Aug 10;255(15):7093–7094. [PubMed] [Google Scholar]
  17. Hammarström S., Murphy R. C., Samuelsson B., Clark D. A., Mioskowski C., Corey E. J. Structure of leukotriene C. Identification of the amino acid part. Biochem Biophys Res Commun. 1979 Dec 28;91(4):1266–1272. doi: 10.1016/0006-291x(79)91203-8. [DOI] [PubMed] [Google Scholar]
  18. Hirsh J., Doery J. C. Platelet function in health and disease. Prog Hematol. 1971;7:185–234. [PubMed] [Google Scholar]
  19. Jakschik B. A., Falkenhein S., Parker C. W. Precursor role of arachidonic acid in release of slow reacting substance from rat basophilic leukemia cells. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4577–4581. doi: 10.1073/pnas.74.10.4577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lewis R. A., Wasserman S. I., Goetzi E. J., Austen K. F. Formation of slow-reacting substance of anaphylaxis in human lung tissue and cells before release. J Exp Med. 1974 Nov 1;140(5):1133–1146. doi: 10.1084/jem.140.5.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mencia-Huerta J. M., Benveniste J. Platelet-activating factor (PAF-acether) and macrophages. II. Phagocytosis-associated release of PAF-acether from rat peritoneal macrophages. Cell Immunol. 1981 Jan 15;57(2):281–292. doi: 10.1016/0008-8749(81)90087-3. [DOI] [PubMed] [Google Scholar]
  22. Morris H. R., Piper P. J., Taylor G. W., Tippins J. R. The role of arachidonate lipoxygenase in the release of SRS-A from guinea-pig chopped lung. Prostaglandins. 1980 Mar;19(3):371–383. doi: 10.1016/0090-6980(80)90071-4. [DOI] [PubMed] [Google Scholar]
  23. Morris H. R., Taylor G. W., Piper P. J., Samhoun M. N., Tippins J. R. Slow reacting substances (SRSs): the structure identification of SRSs from rat basophil leukaemia (RBL-1) cells. Prostaglandins. 1980 Feb;19(2):185–201. doi: 10.1016/0090-6980(80)90019-2. [DOI] [PubMed] [Google Scholar]
  24. Morris H. R., Taylor G. W., Piper P. J., Tippins J. R. Structure of slow-reacting substance of anaphylaxis from guinea-pig lung. Nature. 1980 May 8;285(5760):104–106. doi: 10.1038/285104a0. [DOI] [PubMed] [Google Scholar]
  25. Murphy R. C., Hammarström S., Samuelsson B. Leukotriene C: a slow-reacting substance from murine mastocytoma cells. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4275–4279. doi: 10.1073/pnas.76.9.4275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Orange R. P., Chang P. L. The effect of thiols on immunologic release of slow reacting substance of anaphylaxis. I. Human lung. J Immunol. 1975 Oct;115(4):1072–1077. [PubMed] [Google Scholar]
  27. Orange R. P., Moore E. G. The effect of thiols on the immunologic release of slow reacting substance of anaphylaxis. II. Other in vitro and in vivo models. J Immunol. 1976 Feb;116(2):392–397. [PubMed] [Google Scholar]
  28. Orange R. P., Murphy R. C., Austen K. F. Inactivation of slow reacting substance of anaphylaxins (SRS-A) by arylsulfatases. J Immunol. 1974 Jul;113(1):316–322. [PubMed] [Google Scholar]
  29. Orange R. P., Murphy R. C., Karnovsky M. L., Austen K. F. The physicochemical characteristics and purification of slow-reacting substance of anaphylaxis. J Immunol. 1973 Mar;110(3):760–770. [PubMed] [Google Scholar]
  30. Parker C. W., Huber M. M., Hoffman M. K., Falkenhein S. F. Characterization of the two major species of slow reacting substance from rat basophilic leukemia cells as glutathionyl thioethers of eicosatetraenoic acids oxygenated at the 5 position. Evidence that peroxy groups are present and important for spasmogenic activity. Prostaglandins. 1979 Nov;18(5):673–686. doi: 10.1016/0090-6980(79)90088-1. [DOI] [PubMed] [Google Scholar]
  31. Parker C. W. Prostaglandins and slow-reacting substance. J Allergy Clin Immunol. 1979 Jan;63(1):1–14. doi: 10.1016/0091-6749(79)90155-6. [DOI] [PubMed] [Google Scholar]
  32. Siraganian R. P., Osler A. G. Destruction of rabbit platelets in the allergic response of sensitized leukocytes. I. Demonstration of a fluid phase intermediate. J Immunol. 1971 May;106(5):1244–1251. [PubMed] [Google Scholar]
  33. Stechschulte D. J., Austen K. F., Bloch K. J. Antibodies involved in antigen-induced release of slow reacting substance of anaphylaxis (SRS-A) in the guinea pig and rat. J Exp Med. 1967 Jan 1;125(1):127–147. doi: 10.1084/jem.125.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Vargaftig B. B., Lefort J., Chignard M., Benveniste J. Platelet-activating factor induces a platelet-dependent bronchoconstriction unrelated to the formation of prostaglandin derivatives. Eur J Pharmacol. 1980 Jul 25;65(2-3):185–192. doi: 10.1016/0014-2999(80)90391-x. [DOI] [PubMed] [Google Scholar]
  35. Vargaftig B. B., Tranier Y., Chignard M. Inhibition by sulfhydryl agents of arachidonic acid-induced platelet aggregation and release of potential inflammatory substances. Prostaglandins. 1974 Oct 25;8(2):133–156. doi: 10.1016/0090-6980(74)90076-8. [DOI] [PubMed] [Google Scholar]
  36. Yecies L. D., Wedner H. J., Parker C. W. Slow reacting substance (SRS) from ionophore A23187-stimulated human leukemic basophils. I. Evidence for a precursor role of arachidonic acid and initial purification. J Immunol. 1979 Dec;123(6):2814–2816. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES