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. 1982 Mar;69(3):643–650. doi: 10.1172/JCI110491

Methionine-enkephalin and leucine-enkephalin in human sympathoadrenal system and pheochromocytoma.

T Yoshimasa, K Nakao, H Ohtsuki, S Li, H Imura
PMCID: PMC371021  PMID: 7061706

Abstract

To elucidate the physiological and pathophysiological significance of methionine- and leucine-enkephalin (Met-and Leu-enkephalin, respectively) in human sympathoadrenal system, the contents of these peptides in normal human sympathetic nervous system, adrenal medulla, and pheochromocytomas were determined by specific radioimmunoassays combined with reverse-phase high-performance liquid chromatography. Met-enkephalin-LI and Leu-enkephalin-LI, respectively) were detected by radioimmunoassay in adrenal glands, adrenal medulla, stellate ganglia, sympathetic trunks, and celiac ganglia, and their contents in adrenal medulla were highest. Existence of authentic Met- and Leu-enkephalin was confirmed by reverse-phase high-performance liquid chromatography. Met-enkephalin was approximately 74% of Met-enkephalin-LI, whereas Leu-enkephalin was approximately 30% of Leu-enkephalin-LI in human adrenal medulla. The ratio of Met- to Leu-enkephalin was 2.6 in human adrenal medulla, whereas it was higher in sympathetic ganglia or trunks. In four cases of pheochromocytoma marked difference in Met- and Leu-enkephalin contents was found between medullary and extramedullary tumors. The contents were about three orders higher and the Met- to Leu-enkephalin ratio was lower in medullary than in extramedullary pheochromocytomas, reflecting the tissues where the tumors arose. These results suggest the physiological roles of Met- and Leu-enkephalin in sympathetic nervous system and adrenal glands and their pathophysiological significances in pheochromocytomas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clement-Jones V., Corder R., Lowry P. J. Isolation of human met-enkephalin and two groups of putative precursors (2K-pro-met-enkephalin) from an adrenal medullary tumour. Biochem Biophys Res Commun. 1980 Jul 31;95(2):665–673. doi: 10.1016/0006-291x(80)90837-2. [DOI] [PubMed] [Google Scholar]
  2. Clement-Jones V., Lowry P. J., Rees L. H., Besser G. M. Met-enkephalin circulates in human plasma. Nature. 1980 Jan 17;283(5744):295–297. doi: 10.1038/283295a0. [DOI] [PubMed] [Google Scholar]
  3. Di Giulio A. M., Yang H. Y., Fratta W., Costa E. Decreased content of immunoreactive enkephalin-like peptide in peripheral tissues of spontaneously hypertensive rats. Nature. 1979 Apr 12;278(5705):646–647. doi: 10.1038/278646a0. [DOI] [PubMed] [Google Scholar]
  4. Di Giulio A. M., Yang H. Y., Lutold B., Fratta W., Hong J., Costa E. Characterization of enkephalin-like material extracted from sympathetic ganglia. Neuropharmacology. 1978 Nov;17(11):989–992. doi: 10.1016/0028-3908(78)90144-2. [DOI] [PubMed] [Google Scholar]
  5. Frederickson R. C. Enkephalin pentapeptides--a review of current evidence for a physiological role in vertebrate neurotransmission. Life Sci. 1977 Jul 1;21(1):23–42. doi: 10.1016/0024-3205(77)90421-0. [DOI] [PubMed] [Google Scholar]
  6. Gros C., Pradelles P., Rouget C., Bepoldin O., Dray F., Fournie-Zaluski M. C., Roques B. P., Pollard H., Llorens-Cortes C., Schwartz J. C. Radioimmunoassay of methionine- and leucine-enkephalins in regions of rat brain and comparison with endorphins estimated by a radioreceptor assay. J Neurochem. 1978 Jul;31(1):29–39. doi: 10.1111/j.1471-4159.1978.tb12429.x. [DOI] [PubMed] [Google Scholar]
  7. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  8. Hexum T. D., Yang H. Y., Costa E. Biochemical characterization of enkephalin-like immunoreactive peptides of adrenal glands. Life Sci. 1980 Sep 29;27(13):1211–1216. doi: 10.1016/0024-3205(80)90474-9. [DOI] [PubMed] [Google Scholar]
  9. Hughes J., Kosterlitz H. W., Smith T. W. The distribution of methionine-enkephalin and leucine-enkephalin in the brain and peripheral tissues. Br J Pharmacol. 1977 Dec;61(4):639–647. doi: 10.1111/j.1476-5381.1977.tb07557.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
  11. Lewis R. V., Stern A. S., Kimura S., Rossier J., Stein S., Udenfriend S. An about 50,000-dalton protein in adrenal medulla: a common precursor of [Met]- and [Leu]enkephalin. Science. 1980 Jun 27;208(4451):1459–1461. doi: 10.1126/science.7384787. [DOI] [PubMed] [Google Scholar]
  12. Lundberg J. M., Hamberger B., Schultzberg M., Hökfelt T., Granberg P. O., Efendić S., Terenius L., Goldstein M., Luft R. Enkephalin- and somatostatin-like immunoreactivities in human adrenal medulla and pheochromocytoma. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4079–4083. doi: 10.1073/pnas.76.8.4079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakao K., Yoshimasa T., Oki S., Tanaka I., Nakai Y., Wakimasu M., Fujino M., Imura H. Presence of dynorphin-like immunoreactivity in rat pituitary gland and hypothalamus. Regul Pept. 1981 Jun;2(3):201–208. doi: 10.1016/0167-0115(81)90014-8. [DOI] [PubMed] [Google Scholar]
  14. Ryder S. W., Eng J. Radioimmunoassay of leucine-enkephalin-like substance in human and canine plasma. J Clin Endocrinol Metab. 1981 Feb;52(2):367–369. doi: 10.1210/jcem-52-2-367. [DOI] [PubMed] [Google Scholar]
  15. Schultzberg M., Hökfelt T., Terenius L., Elfvin L. G., Lundberg J. M., Brandt J., Elde R. P., Goldstein M. Enkephalin immunoreactive nerve fibres and cell bodies in sympathetic ganglia of the guinea-pig and rat. Neuroscience. 1979;4(2):249–270. doi: 10.1016/0306-4522(79)90087-3. [DOI] [PubMed] [Google Scholar]
  16. Schultzberg M., Lundberg J. M., Hökfelt T., Terenius L., Brandt J., Elde R. P., Goldstein M. Enkephalin-like immunoreactivity in gland cells and nerve terminals of the adrenal medulla. Neuroscience. 1978;3(12):1169–1186. doi: 10.1016/0306-4522(78)90137-9. [DOI] [PubMed] [Google Scholar]
  17. Sullivan S. N., Bloom S. R., Polak J. M. Enkephalin in peripheral neuroendocrine tumours. Lancet. 1978 May 6;1(8071):986–987. doi: 10.1016/s0140-6736(78)90271-4. [DOI] [PubMed] [Google Scholar]
  18. Yang H. Y., Di Giulio A. M., Fratta W., Hong J. S., Majane E. A., Costa E. Enkephalin in bovine adrenal gland: multiple molecular forms of [Met5]-enkephalin immunoreactive peptides. Neuropharmacology. 1980 Feb;19(2):209–215. doi: 10.1016/0028-3908(80)90140-9. [DOI] [PubMed] [Google Scholar]
  19. Yoshimasa T., Nakao K., Oki S., Tanaka I., Nakai Y., Imura H. Presence of dynorphin-like immunoreactivity in pheochromocytomas. J Clin Endocrinol Metab. 1981 Jul;53(1):213–214. doi: 10.1210/jcem-53-1-212. [DOI] [PubMed] [Google Scholar]

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