Table 2.

mRNA levels of selected genes involved in heart development in P19CL6 cell lines with and without induction to generate a cardiomyocyte phenotype¹.

Gene	Cell line2	mRNA level: noninduced cells (U)	mRNA level: induced cells (I)	I/U3
SRF	Mock clones #1, 2, 3	1.08 ± 0.11	0.46 ± 0.08	0.43 ± 0.01
	mRhoAN19 clone #1	0.60 ± 0.12	1.68 ± 0.11*	2.75 ± 0.06*
Cardiac α-actin	Mock clones #1, 2, 3	0.97 ± 0.04	0.63 ± 0.17	0.65 ± 0.02
	mRhoAN19 clone #1	1.14 ± 0.14	2.93 ± 0.35*	2.57 ± 0.04*
GATA4	Mock clones #1, 2, 3	1.00 ± 0.48	0.35 ± 0.05	0.35 ± 0.04
	mRhoAN19 clone #1	0.70 ± 0.12	2.41 ± 0.38*	3.44 ± 0.09*

¹Data represent the results of 3–6 independent measurements.

²Values for mock transfected (Mock) clones #1, 2, 3 represent combined results for these three cell lines.

³I/U is the ratio of mRNA levels in induced (I) and uninduced (U) cells.

*P < 0.05, compared to mock transfected cell lines.