Fig. 8.

Evaluation of androgen-induced activities of the wild type –286PBCAT, mtTS1/−286PBCAT and mtTS2/−286PBCAT constructs in LNCaP cells. (A) Schematic representation of the wild type –286PBCAT, mtTS/1-286PBCAT and mtTS2/−286PBCAT constructs. (B) These constructs were cotransfected with AR and Renilla luciferase expression vectors into LNCaP cells and treated with or without 10⁻⁸ M DHT for 24 hours before harvest. Background activities of cell lysates with no DNA transfection were subtracted from the data contained in the experimental group, before the normalization with Renilla luciferase activities. The activity induction in response to hormone treatment was determined by comparing receptor gene activity induced by DHT to the corresponding baseline values in the absence of hormone. Data shown here are a representative from at least three independent experiments in triplicate. Error bars indicate SD values. Results are presented as relative CAT activities dpm/min/mg protein.