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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Bone. 2013 Jan 19;53(2):478–486. doi: 10.1016/j.bone.2013.01.020

Figure 1. Effect of polyphosphates on MC3T3-E1 osteoblast culture mineralization.

Figure 1

MC3T3-E1 osteoblast cells cultured in 50 μg/ml ascorbic acid, 10 mM β-glycerophosphate, and (A) PolyP5 or (B) PolyP65 for 12 days. Mineralization in the culture plates was visualized by von Kossa staining (black) and mineralization was quantified using ImageJ. Both PolyPs dose-dependently inhibit mineralization. Data are presented as mean values ± SE. ***p<0.001, Student's t-test relative to the 0 μM PolyP treatment.