Abstract
Conditioned media prepared from human lung, placenta, peripheral leukocytes, cultured human pancreatic carcinoma cells, and cultured cervical carcinoma cells exhibit a common pattern of two distinct colony-stimulating factors (CSF) separable by isoelectrofocusing. Type I CSF appears polydisperse on isoelectrofocusing, showing multiple peaks with an isoelectric point of 3.6-4.7 and mol wt 50,000. It has a much higher activity in mouse than in human bone marrow, and stimulates the formation of predominately macrophage colonies. Type II CSF has an isoelectric point of 5.7 and mol wt 27,000 and exhibits higher activity in human than in mouse marrow, yielding predominately granulocytic colonies.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burgess A. W., Camakaris J., Metcalf D. Purification and properties of colony-stimulating factor from mouse lung-conditioned medium. J Biol Chem. 1977 Mar 25;252(6):1998–2003. [PubMed] [Google Scholar]
- Fojo S. S., Wu M. C., Gross M. A., Purcell Y., Yunis A. A. Purification and characterization of a colony stimulating factor from human lung. Biochemistry. 1978 Jul 25;17(15):3109–3116. doi: 10.1021/bi00608a026. [DOI] [PubMed] [Google Scholar]
- Fojo S. S., Wu M. C., Gross M. A., Yunis A. A. The isolation and characterization of a colony stimulating factor from human lung. Biochim Biophys Acta. 1977 Sep 27;494(1):92–99. doi: 10.1016/0005-2795(77)90137-4. [DOI] [PubMed] [Google Scholar]
- Guez M., Sachs L. Purification of the protein that induces cell differentiation to macrophages and granulocytes. FEBS Lett. 1973 Dec 1;37(2):149–154. doi: 10.1016/0014-5793(73)80446-6. [DOI] [PubMed] [Google Scholar]
- Kurland J. I., Bockman R. S., Broxmeyer H. E., Moore M. A. Limitation of excessive myelopoiesis by the intrinsic modulation of macrophage-derived prostaglandin E. Science. 1978 Feb 3;199(4328):552–555. doi: 10.1126/science.304600. [DOI] [PubMed] [Google Scholar]
- Stanley E. R. Colony-stimulating factor (CSF) radioimmunoassay: detection of a CSF subclass stimulating macrophage production. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2969–2973. doi: 10.1073/pnas.76.6.2969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanley E. R., Hansen G., Woodcock J., Metcalf D. Colony stimulating factor and the regulation of granulopoiesis and macrophage production. Fed Proc. 1975 Dec;34(13):2272–2278. [PubMed] [Google Scholar]
- Stanley E. R., Heard P. M. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. J Biol Chem. 1977 Jun 25;252(12):4305–4312. [PubMed] [Google Scholar]
- Waheed A., Shadduck R. K. Purification and properties of L cell-derived colony-stimulating factor. J Lab Clin Med. 1979 Jul;94(1):180–193. [PubMed] [Google Scholar]
- Wu M., Arimura G. K., Yunis A. A. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry. 1977 May 3;16(9):1908–1913. doi: 10.1021/bi00628a023. [DOI] [PubMed] [Google Scholar]
- Wu M., Cini J. K., Yunis A. A. Purification of a colony-stimulating factor from cultured pancreatic carcinoma cells. J Biol Chem. 1979 Jul 25;254(14):6226–6228. [PubMed] [Google Scholar]
- Yunis A. A., Arimura G. K., Russin D. J. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int J Cancer. 1977 Jan;19(1):128–135. doi: 10.1002/ijc.2910190118. [DOI] [PubMed] [Google Scholar]