Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1980 Dec;66(6):1428–1431. doi: 10.1172/JCI109997

Potassium and angiotensin II increase the concentrations of phosphatidic acid, phosphatidylinositol, and polyphosphoinositides in rat adrenal capsules in vitro.

R V Farese, M A Sabir, R E Larson
PMCID: PMC371630  PMID: 6255013

Abstract

We examined the effects of K+ and angiotensin II, the major regulators of aldosterone secretion, on phospholipid metabolism during incubation of glomerulosa-rich, adrenal capsules. Addition of increasing amounts of K+ and angiotensin II to the incubation media elicited progressive increases in corticosterone production and capsular concentrations of phosphatidic acid, phosphatidyl-inositol, and polyphosphoinositides. These effects are similar to those previously reported for ACTH in the whole adrenal cortex. A common mechanism, i.e., activation of the phosphatidate-polyphosphoinositide cycle, may be operative in the action of steroidogenic agents in their target tissues.

Full text

PDF
1428

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguilera G., Catt K. J. Regulation of aldosterone secretion by the renin-angiotensin system during sodium restriction in rats. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4057–4061. doi: 10.1073/pnas.75.8.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albano J. D., Brown B. L., Ekins R. P., Tait S. A., Tait J. F. The effects of potassium, 5-hydrocytryptamine, adrenocorticotrophin and angiotensin II on the concentration of adenosine 3':5'-cyclic monophosphate in suspensions of dispersed rat adrenal zona glomerulosa and zona fasciculata cells. Biochem J. 1974 Aug;142(2):391–400. doi: 10.1042/bj1420391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brownie A. C., Alfano J., Jefcoate C. R., Orme-Johnson W., Beinert H., Simpson E. R. Effect of ACTH on adrenal mitochondrial cytochrome P-450 in the rat. Ann N Y Acad Sci. 1973;212:344–360. doi: 10.1111/j.1749-6632.1973.tb47606.x. [DOI] [PubMed] [Google Scholar]
  4. DAVIS J. O., URQUHART J., HIGGINS J. T., Jr The effects of alteration of plasma sodium and potassium concentration on aldosterone secretion. J Clin Invest. 1963 May;42:597–609. doi: 10.1172/JCI104750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FARESE R. V. INHIBITION OF THE STEROIDOGENIC EFFECT OF ACTH AND INCORPORATION OF AMINO ACID INTO RAT ADRENAL PROTEIN IN VITRO BY CHLORAMPHENICOL. Biochim Biophys Acta. 1964 Aug 12;87:699–701. doi: 10.1016/0926-6550(64)90292-0. [DOI] [PubMed] [Google Scholar]
  6. FERGUSON J. J., Jr PROTEIN SYNTHESIS AND ADRENOCORTICOTROPIN RESPONSIVENESS. J Biol Chem. 1963 Aug;238:2754–2759. [PubMed] [Google Scholar]
  7. Farese R. V., Prudente W. J., Chuang L. T. Non-esterified cholesterol-rich adrenal lipid fractions. Preparation, properties and preferential utilization for cholesterol side-chain cleavage by corticotropin-stimulated adrenal mitochondria. Biochem J. 1980 Jan 15;186(1):145–152. doi: 10.1042/bj1860145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farese R. V., Prudente W. J. On the role of intra-adrenal unesterified cholesterol in the steroidogenic effect of corticotropin. Biochim Biophys Acta. 1978 Nov 15;544(1):77–84. doi: 10.1016/0304-4165(78)90211-8. [DOI] [PubMed] [Google Scholar]
  9. Farese R. V., Sabir A. M. Polyphosphoinositides:stimulator of mitochondrial cholesterol side chain cleavage and possible identification as an adrenocorticotropin-induced, cycloheximide-sensitive, cytosolic, steroidogenic factor. Endocrinology. 1980 Jun;106(6):1869–1879. doi: 10.1210/endo-106-6-1869. [DOI] [PubMed] [Google Scholar]
  10. Farese R. V., Sabir A. M., Vandor S. L. Adrenocorticotropin acutely increases adrenal polyphosphoinositides. J Biol Chem. 1979 Aug 10;254(15):6842–6844. [PubMed] [Google Scholar]
  11. Farese R. V., Sabir A. M., Vandor S. L., Larson R. E. Are polyphosphoinositides the cycloheximide-sensitive mediator in the steroidogenic actions of adrenocorticotropin and adenosine-3',5'-monophosphate? J Biol Chem. 1980 Jun 25;255(12):5728–5734. [PubMed] [Google Scholar]
  12. Farese R. V., Sabir M. A., Larson R. E. On the mechanism whereby ACTH and cyclic AMP increase adrenal polyphosphoinositides. Rapid stimulation of the synthesis of phosphatidic acid and derivatives of CDP - diacylglycerol. J Biol Chem. 1980 Aug 10;255(15):7232–7237. [PubMed] [Google Scholar]
  13. Fujita K., Aguilera G., Catt K. J. The role of cyclic AMP in aldosterone production by isolated zona glomerulosa cells. J Biol Chem. 1979 Sep 10;254(17):8567–8574. [PubMed] [Google Scholar]
  14. Garren L. D., Ney R. L., Davis W. W. Studies on the role of protein synthesis in the regulation of corticosterone production by adrenocorticotropic hormone in vivo. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1443–1450. doi: 10.1073/pnas.53.6.1443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ichii S. Acute effect of corticotropin on the incorporation of 3H-choline into phospholipid fraction of subcellular components from rat adrenal glands. Endocrinol Jpn. 1970 Dec;17(6):517–520. doi: 10.1507/endocrj1954.17.517. [DOI] [PubMed] [Google Scholar]
  16. LARAGH J. H., ANGERS M., KELLY W. G., LIEBERMAN S. Hypotensive agents and pressor substances. The effect of epinephrine, norepinephrine, angiotensin II, and others on the secretory rate of aldosterone in man. JAMA. 1960 Sep 17;174:234–240. doi: 10.1001/jama.1960.03030030014003. [DOI] [PubMed] [Google Scholar]
  17. SILBER R. H., BUSCH R. D., OSLAPAS R. Practical procedure for estimation of corticosterone or hydrocortisone. Clin Chem. 1958 Aug;4(4):278–285. [PubMed] [Google Scholar]
  18. STONE D., HECHTER O. Studies on ACTH action in perfused bovine adrenals: the site of action of ACTH in corticosteroidogenesis. Arch Biochem Biophys. 1954 Aug;51(2):457–469. doi: 10.1016/0003-9861(54)90501-9. [DOI] [PubMed] [Google Scholar]
  19. Simpson E. R., Jefcoate C. R., Brownie A. C., Boyd G. S. The effect of ether anaesthesia stress on cholesterol-side-chain cleavage and cytochrome P450 in rat-adrenal mitochondria. Eur J Biochem. 1972 Jul 24;28(3):442–450. doi: 10.1111/j.1432-1033.1972.tb01931.x. [DOI] [PubMed] [Google Scholar]
  20. Simpson E. R., McCarthy J. L., Peterson J. A. Evidence that the cycloheximide-sensitive site of adrenocorticotropic hormone action is in the mitochondrion. Changes in pregnenolone formation, cholesterol content, and the electron paramagnetic resonance spectra of cytochrome P-450. J Biol Chem. 1978 May 10;253(9):3135–3139. [PubMed] [Google Scholar]
  21. Williams G. H., McDonnell L. M., Tait S. A., Tait J. F. The effect of medium composition and in vitro stimuli on the conversion of corticosterone to aldosterone in rat glomerulosa tissue. Endocrinology. 1972 Oct;91(4):948–960. doi: 10.1210/endo-91-4-948. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES