Figure 4.
Flow cytometric analysis of the MNCs in the liver and spleen of dnTGFβRII, OT-I/dnTGFβRII/Rag1-/- (OT-I/ dnTGFβRII), and OT-I/Rag1-/- (OT-I/B6) mice. (A) Number and phenotype of total MNCs and CD8+ T cells in the spleen and liver of dnTGFβRII mice (n=7), OT-I/ dnTGFβRII (n=8), and OT-I/B6 (n=8) mice. (B) The frequency of IFNγ producing CD8+ T cells in the spleen of dnTGFβRII (n=4), OT-I/dnTGFβRII (n=4), and OT-I/B6 (n=4) mice. The cells were stimulated ex vivo with anti-CD3 and anti-CD28 (left panel) or the OVA peptide 257-264 (right panel). *p<0.05, **p<0.01, ***p<0.001 determined using one-way ANOVA followed by a Bonferroni multiple comparisons test or Kruskal-Wallis test followed by Dunn's multiple comparisons test.