Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1978 Aug;62(2):367–372. doi: 10.1172/JCI109137

Multiple forms of human plasma renin substrate.

P Eggena, H Hidaka, J D Barrett, M P Sambhi
PMCID: PMC371774  PMID: 670398

Abstract

The objective of this investigation was to determine whether heterogeneity of plasma renin substrate could be observed in states of steroid excess and various forms of hypertensive disease. In states of stimulated renin substrate production by estrogens or glucocorticoids, multiple forms of renin substrate were apparent when stimulation was excessive. Stimulation of substrate production caused by uremia associated with hypertension showed similar results. None, or only trace quantities of the additional forms of renin substrate were evident in subjects with normal or suppressed levels of plasma renin substrate. The additional forms of renin substrate could be distinguished from the normal form on the basis of cross-reactivity with a specific antiserum to the normal form, electrophoretic mobility, and kinetic rate constants. Differences in rate constants of the various forms of plasma renin substrate may account for the altered rate of the renin reaction associated with several states of hypertension. In plasma of patients with renovascular hypertension, significant quantities of a protein which cross-reacted with the antiserum but could not generate angiotensin I were observed.

Full text

PDF
367

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett J. D., Eggena P., Sambhi M. P. Influence of angiotensinase ihhibitors on the enzymatic activity of renin. Biochem Med. 1976 Oct;16(2):157–168. doi: 10.1016/0006-2944(76)90019-3. [DOI] [PubMed] [Google Scholar]
  2. Cain M. D., Walters W. A., Catt K. J. Effects of oral contraceptive therapy on the renin-angiotensin system. J Clin Endocrinol Metab. 1971 Oct;33(4):671–676. doi: 10.1210/jcem-33-4-671. [DOI] [PubMed] [Google Scholar]
  3. Eggena P., Barrett J. D., Hidaka H., Chu C. L., Thananopavarn C., Golub M. S., Sambhi M. P. A direct radioimmunoassay for human renin substrate and identification of multiple substrate types in plasma. Circ Res. 1977 Oct;41(4 Suppl 2):34–37. doi: 10.1161/01.res.41.4.34. [DOI] [PubMed] [Google Scholar]
  4. Eggena P., Barrett J. D., Sambhi M. P., Wiedeman C. E. The validity of comparing the measurements of angiotensin I generated in human plasma by radioimmunoassay and bioassay. J Clin Endocrinol Metab. 1974 Nov;39(5):865–870. doi: 10.1210/jcem-39-5-865. [DOI] [PubMed] [Google Scholar]
  5. Eggena P., Chu C. L., Barrett J. D., Sambhi M. P. Purification and partial characterization of human angiotensinogen. Biochim Biophys Acta. 1976 Mar 18;427(1):208–217. doi: 10.1016/0005-2795(76)90297-x. [DOI] [PubMed] [Google Scholar]
  6. Gordon D. B., Sachin I. N., Dodd V. N. Heterogeneity of renin substrate in human plasma: effect of pregnancy and oral contraceptives. Proc Soc Exp Biol Med. 1976 Nov;153(2):314–318. doi: 10.3181/00379727-153-39536. [DOI] [PubMed] [Google Scholar]
  7. Haas E., Goldblatt H., Gipson E. C., Lewis L. Extraction, purification, and assay of human renin free of angiotensinase. Circ Res. 1966 Oct;19(4):739–749. doi: 10.1161/01.res.19.4.739. [DOI] [PubMed] [Google Scholar]
  8. Lim R., Tadayyon E. Fractionation of the insoluble brain proteins with acrylamide electrophoresis. Anal Biochem. 1970 Mar;34:9–15. doi: 10.1016/0003-2697(70)90080-1. [DOI] [PubMed] [Google Scholar]
  9. Nasjletti A., Lewis L. A., Masson G. M. Electrophoretic studies on renin substrate from various species. Am J Physiol. 1971 Mar;220(3):804–807. doi: 10.1152/ajplegacy.1971.220.3.804. [DOI] [PubMed] [Google Scholar]
  10. Printz M. P., Printz J. M., Lewicki J. A., Gregory T. Resolution of multiple forms of human angiotensinogen. Isoelectric focusing heterogeneity profile analysis. Circ Res. 1977 Oct;41(4 Suppl 2):37–43. doi: 10.1161/01.res.41.4.37. [DOI] [PubMed] [Google Scholar]
  11. Sambhi M. P., Eggena P., Barrett J. C., Tuck M., Wiedeman C. E., Thananopavarn C. A circulating renin activator in essential hypertension. Circ Res. 1975 Jun;36(6 Suppl 1):28–37. doi: 10.1161/01.res.36.6.28. [DOI] [PubMed] [Google Scholar]
  12. Sambhi M. P., Wiedeman C. E. Renin activation in the venous plasma from the involved kidney in the patient with renal hypertension. J Clin Invest. 1972 Jan;51(1):22–30. doi: 10.1172/JCI106792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weinberger M. H., Collins R. D., Dowdy A. J., Nokes G. W., Luetscher J. A. Hypertension induced by oral contraceptives containing estrogen and gestagen. Effects on plasma renin activity and aldosterone excretion. Ann Intern Med. 1969 Nov;71(5):891–902. doi: 10.7326/0003-4819-71-5-891. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES