Table 2. Common Integration Sites for Feral strains of MMTV in Mouse Mammary Tumors1.
| Gene | Chr. | #insert. Events | CzechII HOGs | HOG Tumors | CzechII | Balb/cf MMTV(CZ) | Balb/cf MMTV(SP) | MA #1 | MA #2 | RT-PCR |
|---|---|---|---|---|---|---|---|---|---|---|
| Phf19 | 2 | 3 | 0 | 0 | 2 | 1 | 0 | N | N | Y |
| Sfmbt2 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | NT | Y | Y |
| Fgr | 4 | 2 | 0 | 0 | 0 | 0 | 2 | NT | Y | Y |
| Gm12690 | 4 | NT | NT | NT | ||||||
| Gm3917 | 4 | 3 | 0 | 0 | 2 | 1 | 0 | NT | NT | NT |
| Gm12684 | 4 | NT | NT | NT | ||||||
| Pdgfra | 5 | 3 | 1 | 2 | 0 | 0 | 0 | Y | NT | Y |
| Fezf1 | 6 | 3 | 0 | 0 | 2 | 1 | 0 | Y | Y | Y |
| Tacstd2 | 6 | 2 | 0 | 0 | 0 | 1 | 1 | NT | Y | Y |
| Fgf15 | 7 | Y | Y | Y | ||||||
| Fgf3 | 7 | 17 | 0 | 1 | 7 | 2 | 7 | Y | Y | Y |
| Fgf4 | 7 | Y | Y | Y | ||||||
| Foxl1 | 8 | 2 | 0 | 0 | 0 | 2 | 0 | NT | NT | Y |
| Gm6856 | 8 | 2 | 0 | 0 | 1 | 0 | 1 | NT | NT | NT |
| Rspo3 | 10 | 4 | 0 | 0 | 1 | 1 | 2 | NT | NT | Y |
| Gm7000 | 10 | 5 | 0 | 0 | 0 | 3 | 2 | NT | NT | NT |
| Gm8613 | 10 | 2 | 0 | 0 | 0 | 1 | 1 | NT | NT | NT |
| Gm2019 | 13 | 2 | 0 | 0 | 0 | 1 | 1 | NT | NT | NT |
| Gm5770 | 14 | 2 | 0 | 0 | 0 | 1 | 1 | NT | NT | NT |
| Wnt10b | 15 | Y | Y | Y | ||||||
| Wnt1 | 15 | 20 | 1 | 5 | 5 | 9 | Y | Y | Y | |
| Ddn | 15 | Y | Y | Y | ||||||
| Rspo2 | 15 | 22 | 10 | 1 | 5 | 1 | 5 | Y | Y | Y |
| Sdc2 | 15 | 4 | 0 | 0 | 1 | 0 | 3 | Y | Y | Y |
| Npm3 | 19 | Y | NT | Y | ||||||
| Fgf8 | 19 | 5 | 0 | 0 | 3 | 1 | 1 | Y | NT | Y |
| Col4a5 | X | 2 | 0 | 1 | 0 | 0 | 1 | Y | NT | Y |
1
The number of MMTV insertion events is indicated and in which tumor panels they occurred. Genes underlined comprise a cluster of genes that are affected by MMTV integration. In a cluster of genes the number of insertion events is given for only one gene in the cluster. MA#1 and MA#2 represent the results of two independent microarray analysis of mammary tumor RNAs. The results can be visualized at the website [16]. RT-PCR summarizes the results of gel analysis of RT-PCR products shown in Supplemental Figures 1 and 2. N= not up regulated with respect to mammary gland from 15 day pregnant mice, Y= yes up regulated with respect to mammary gland from 15 day pregnant mice, NT=not tested, Chr.= chromosome number