Abstract
The influence of 17beta-estradiol and 17alpha-estradiol on adult human omental adipocyte precursors grown in a propagating culture system was studied. Cells were grown in subculture in the presence or absence of hormone. 17beta-estradiol resulted in significant promotion of adipocyte precursor replication, as determined by cell counting and incorporation of radioactive thymidine into DNA. The hormone stimulated cell multiplication in the concentration range 0.5--500 ng/ml growth medium. The highest level tested was 500 ng/ml. The maximal effects were obtained at 50 ng/ml (P less than 0.001 by paired t test, 48 h after hormone addition). All 10 cell strains (five were derived from men and five from women) that were tested responded similarly to the hormone. 17beta-estradiol did not affect cell size. 17alpha-estradiol did not promote the replication of adipocyte precursors, nor did it influence cell size. Thus, 17beta-estradiol, which is the active isomer in known target tissues, stimulates the multiplication of human adipocyte precursors in culture.
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Selected References
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- Bleau G., Roberts K. D., Chapdelaine A. The in vitro and in vivo uptake and metabolism of steroids in human adipose tissue. J Clin Endocrinol Metab. 1974 Aug;39(2):236–246. doi: 10.1210/jcem-39-2-236. [DOI] [PubMed] [Google Scholar]
- Chan L., Jackson R. L., O'Malley B. W., Means A. R. Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen. J Clin Invest. 1976 Aug;58(2):368–379. doi: 10.1172/JCI108481. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan L., O'Malley B. W. Mechanism of action of the sex steroid hormones (first of three parts). N Engl J Med. 1976 Jun 10;294(24):1322–1328. doi: 10.1056/NEJM197606102942405. [DOI] [PubMed] [Google Scholar]
- Chen L., Lindner H. R., Lancet M. Mitogenic action of oestradiol-17 beta on human myometrial and endometrial cells in long-term tissue cultures. J Endocrinol. 1973 Oct;59(1):87–97. doi: 10.1677/joe.0.0590087. [DOI] [PubMed] [Google Scholar]
- GARN S. M., HASKELL J. A. Fat changes during adolescence. Science. 1959 Jun 12;129(3363):1615–1616. doi: 10.1126/science.129.3363.1615. [DOI] [PubMed] [Google Scholar]
- Hiratsu T. In vitro cultivation of human endometrium and the influences of steroid hormones on a cell line derived from the endometrium. Kobe J Med Sci. 1968 Mar;14(1):29–48. [PubMed] [Google Scholar]
- Kalimi M., Tsai S. Y., Tsai M. J., Clark J. H., O'Malley B. W. Effect of estrogen on gene expression in the chick oviduct. Correlation between nuclear-bound estrogen receptor and chromatin initiation site for transcription. J Biol Chem. 1976 Jan 25;251(2):516–523. [PubMed] [Google Scholar]
- Katzenellenbogen B. S., Gorski J. Estrogen action in vitro. Induction of the synthesis of a specific uterine protein. J Biol Chem. 1972 Feb 25;247(4):1299–1305. [PubMed] [Google Scholar]
- Krotkiewski M. The effects of estrogens on regional adipose tissue cellularity in the rat. Acta Physiol Scand. 1976 Jan;96(1):128–133. doi: 10.1111/j.1748-1716.1976.tb10178.x. [DOI] [PubMed] [Google Scholar]
- Lindner H. R., Perel E., Friedlander A., Zeitlin A. Specificity of antibodies to ovarian hormones in relation to the steroid hapten to the peptide carrier. Steroids. 1972 Mar;19(3):357–375. doi: 10.1016/0039-128x(72)90076-1. [DOI] [PubMed] [Google Scholar]
- Longcope C., Pratt J. H., Schneider S. H., Fineberg S. E. In vivo studies on the metabolism of estrogens by muscle and adipose tissue of normal males. J Clin Endocrinol Metab. 1976 Nov;43(5):1134–1145. doi: 10.1210/jcem-43-5-1134. [DOI] [PubMed] [Google Scholar]
- Luskey K. L., Brown M. S., Goldstein J. L. Stimulation of the synthesis of very low density lipoproteins in rooster liver by estradiol. J Biol Chem. 1974 Sep 25;249(18):5939–5947. [PubMed] [Google Scholar]
- MUNKRES K. D., RICHARDS F. M. THE PURIFICATION AND PROPERTIES OF NEUROSPORA MALATE DEHYDROGENASE. Arch Biochem Biophys. 1965 Mar;109:466–479. doi: 10.1016/0003-9861(65)90391-7. [DOI] [PubMed] [Google Scholar]
- O'Malley B. W., Means A. R. Female steroid hormones and target cell nuclei. Science. 1974 Feb 15;183(4125):610–620. doi: 10.1126/science.183.4125.610. [DOI] [PubMed] [Google Scholar]
- Oka T., Schimke R. T. Interaction of estrogen and progesterone in chick oviduct development. II. Effects of estrogen and progesterone on tubular gland cell function. J Cell Biol. 1969 Oct;43(1):123–137. doi: 10.1083/jcb.43.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roncari D. A. Metabolism of estrogens in rat adipose tissue. Endocrinology. 1967 Jun;80(6):1160–1163. doi: 10.1210/endo-80-6-1160. [DOI] [PubMed] [Google Scholar]
- Van R. L., Bayliss C. E., Roncari D. A. Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest. 1976 Sep;58(3):699–704. doi: 10.1172/JCI108516. [DOI] [PMC free article] [PubMed] [Google Scholar]