Abstract
A specific inhibitory activity of in vitro proliferative responses of normal human lymphocytes to Candida metabolic antigen was found in the serum of 6 out of 23 children with chronic mucocutaneous candidiasis. In each of the six patients, the presence of an inhibitory activity was associated with Candida-specific cellular defects, characterized by a negative-skin test and a lack of in vitro lymphocyte proliferation. The presence of a circulating inhibitor was detected during relapses of the disease and disappeared under antifungal therapy. This inhibitory effect was not associated with any toxicity on tested lymphocytes. The factor was shown to be nondialysable, thermostable, nonprecipitable with ammonium sulfate and absorbable on anti-Candida antibodies or concanavalin A-coupled agarose columns. Altogether, these results suggest that the inhibitory factor is not an immunoglobulin, but rather a polysaccharidic antigen of Candida albicans. An inhibition of Candida-induced proliferative response of normal human lymphocytes was also obtained by addition of polysacharide antigens or purified mannans from C. albicans to cultures. Candida polysaccharidic antigens appeared, therefore, to be involved in specific depression of cellular functions observed in chronic candidiasis.
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Selected References
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- Aronson I. K., Soltani K. Chronic mucocutaneous candidosis: a review. Mycopathologia. 1976 Dec 10;60(1):17–25. doi: 10.1007/BF00442543. [DOI] [PubMed] [Google Scholar]
- Axelsen N. H., Kirkpatrick C. H., Buckley R. H. Precipitins to Candida albicans in chronic mucocutaneous candidiasis studied by crossed immunoelectrophoresis with intermediate gel. Correlation with clinical and immunological findings. Clin Exp Immunol. 1974 Jul;17(3):385–394. [PMC free article] [PubMed] [Google Scholar]
- Axelsen N. H., Kirkpatrick C. H. Simultaneous characterization of free Candida antigens and Candida precipitins in a patient's serum by means of crossed immunoelectrophoresis with intermidiate gel. J Immunol Methods. 1973 Apr;2(3):245–249. doi: 10.1016/0022-1759(73)90050-1. [DOI] [PubMed] [Google Scholar]
- Axén R., Ernback S. Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers. Eur J Biochem. 1971 Feb 1;18(3):351–360. doi: 10.1111/j.1432-1033.1971.tb01250.x. [DOI] [PubMed] [Google Scholar]
- Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
- Canales L., Middlemas R. O., 3rd, Louro J. M., South M. A. Immunological observations in chronic mucocutaneous candidiasis. Lancet. 1969 Sep 13;2(7620):567–571. doi: 10.1016/s0140-6736(69)90264-5. [DOI] [PubMed] [Google Scholar]
- HASENCLEVER H. F., MITCHELL W. O. IMMUNOCHEMICAL STUDIES ON POLYSACCHARIDES OF YEASTS. J Immunol. 1964 Nov;93:763–771. [PubMed] [Google Scholar]
- Higgs J. M., Wells R. S. Chronic muco-cutaneous candidiasis: new approaches to treatment. Br J Dermatol. 1973 Aug;89(2):179–190. doi: 10.1111/j.1365-2133.1973.tb02955.x. [DOI] [PubMed] [Google Scholar]
- Kirkpatrick C. H., Rich R. R., Bennett J. E. Chronic mucocutaneous candidiasis: model-building in cellular immunity. Ann Intern Med. 1971 Jun;74(6):955–978. doi: 10.7326/0003-4819-74-6-955. [DOI] [PubMed] [Google Scholar]
- Kirkpatrick C. H., Smith T. K. Chronic mucocutaneous candidiasis: immunologic and antibiotic therapy. Ann Intern Med. 1974 Mar;80(3):310–320. doi: 10.7326/0003-4819-80-3-310. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Longbottom J. L., Brighton W. D., Edge G., Pepys J. Antibodies mediating type I skin test reactions to polysaccharide and protein antigens of Candida albicans. Clin Allergy. 1976 Jan;6(1):41–49. doi: 10.1111/j.1365-2222.1976.tb01410.x. [DOI] [PubMed] [Google Scholar]
- Meister H., Heymer B., Schäfer H., Haferkamp O. Role of Candida albicans in granulomatous tissue reactions. I. In vitro degradation of C. albicans and immunospecificity of split products. J Infect Dis. 1977 Feb;135(2):224–234. doi: 10.1093/infdis/135.2.224. [DOI] [PubMed] [Google Scholar]
- Meister H., Heymer B., Schäfer H., Haferkamp O. Role of Candida albicans in granulomatous tissue reactions. II. In vivo degradation of C. albicans in hepatic macrophages of mice. J Infect Dis. 1977 Feb;135(2):235–242. doi: 10.1093/infdis/135.2.235. [DOI] [PubMed] [Google Scholar]
- Paterson P. Y., Semo R., Blumenschein G., Swelstad J. Mucocutaneous candidiasis, anergy and a plasma inhibitor of cellular immunity: reversal after amphotericin B therapy. Clin Exp Immunol. 1971 Nov;9(5):595–602. [PMC free article] [PubMed] [Google Scholar]
- Stobo J. D., Paul S., Van Scoy R. E., Hermans P. E. Suppressor thymus-derived lymphocytes in fungal infection. J Clin Invest. 1976 Feb;57(2):319–328. doi: 10.1172/JCI108283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takeya K., Nomoto K., Matsumoto T., Miyake T., Himeno K. Chronic mucocutaneous candidiasis accompanied by enhanced antibody production. Clin Exp Immunol. 1976 Sep;25(3):497–500. [PMC free article] [PubMed] [Google Scholar]
- Twomey J. J., Waddell C. C., Krantz S., O'reilly R., L'esperance P., Good R. A. Chronic mucocutaneous candidiasis with macrophage dysfunction, a plasma inhibitor, and co-existent aplastic anemia. J Lab Clin Med. 1975 Jun;85(6):968–977. [PubMed] [Google Scholar]
- Valdimarsson H., Higgs J. M., Wells R. S., Yamamura M., Hobbs J. R., Holt P. J. Immune abnormalities associated with chronic mucocutaneous candidiasis. Cell Immunol. 1973 Mar;6(3):348–361. doi: 10.1016/0008-8749(73)90035-x. [DOI] [PubMed] [Google Scholar]
- Weiner M. H., Yount W. J. Mannan antigenemia in the diagnosis of invasive Candida infections. J Clin Invest. 1976 Nov;58(5):1045–1053. doi: 10.1172/JCI108555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiener E., Bandieri A. Differences in antigen handling by peritoneal macrophages from the Biozzi high and low responder lines of mice. Eur J Immunol. 1974 Jul;4(7):457–463. doi: 10.1002/eji.1830040703. [DOI] [PubMed] [Google Scholar]