Abstract
A fluid-phase immunoradiometric assay has been developed which identifies an antigen on the Factor VIII (antihemophilic factor) procoagulant protein. This sensitive and quantitative assay is not influenced by levels of Favor VIII-related antigen (von Willebrand factor) or other plasma proteins. There is a close correlation of procoagulant activity and immunologically detectable protein in normal and von Willebrand's disease plasmas. In contrast, several different patterns have been identified in hemophilic plasmas. Neither procoagulant activity nor procoagulant antigen is detectable in plasmas from patients with severe classic hemophilia. Patients with mild and moderate hemophilia have either comparable plasma concentrations of procoagulant activity and procoagulant antigen or relatively greater levels of immunologically detectable protein.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biggs R. The absorption of human factor VIII neutralizing antibody by factor VIII. Br J Haematol. 1974 Feb;26(2):259–267. doi: 10.1111/j.1365-2141.1974.tb00471.x. [DOI] [PubMed] [Google Scholar]
- Denson K. W., Biggs R., Haddon M. E., Borrett R., Cobb K. Two types of haemophilia (A+ and A-): a study of 48 cases. Br J Haematol. 1969 Aug;17(2):163–171. doi: 10.1111/j.1365-2141.1969.tb01355.x. [DOI] [PubMed] [Google Scholar]
- Feinstein D., Chong M. N., Kasper C. K., Rapaport S. I. Hemophilia A: polymorphism detectable by a factor 8 antibody. Science. 1969 Mar 7;163(3871):1071–1072. doi: 10.1126/science.163.3871.1071. [DOI] [PubMed] [Google Scholar]
- Hoyer L. W., Breckenridge R. T. Immunologic studies of antihemophilic factor (AHF, factor VIII): cross-reacting material in a genetic variant of hemophilia A. Blood. 1968 Dec;32(6):962–971. [PubMed] [Google Scholar]
- Lazarchick J., Hoyer L. W. The properties of immune complexes formed by human antibodies to factor VIII. J Clin Invest. 1977 Nov;60(5):1070–1079. doi: 10.1172/JCI108858. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peake I. R., Bloom A. L. Immunoradiometric assay of procoagulant factor-VIII antigen in plasma and serum and its reduction in haemophilia. Preliminary studies on adult and fetal blood. Lancet. 1978 Mar 4;1(8062):473–475. doi: 10.1016/s0140-6736(78)90137-x. [DOI] [PubMed] [Google Scholar]
- Poon M. C., Ratnoff O. D. Immunologic evidence that the antihemophilic factor (factor VIII)-like material in hemophilic plasma possesses a nonfunctional low molecular weight subcomponent. Blood. 1977 Sep;50(3):367–376. [PubMed] [Google Scholar]
- Steinbuch M., Audran R. The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys. 1969 Nov;134(2):279–284. doi: 10.1016/0003-9861(69)90285-9. [DOI] [PubMed] [Google Scholar]
- Thompson A. R. Factor IX antigen by radioimmunoassay. Abnormal factor IX protein in patients on warfarin therapy and with hemophilia B. J Clin Invest. 1977 May;59(5):900–910. doi: 10.1172/JCI108712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorell J. I., Johansson B. G. Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta. 1971 Dec 28;251(3):363–369. doi: 10.1016/0005-2795(71)90123-1. [DOI] [PubMed] [Google Scholar]
- Zimmerman T. S., de la Pointe L., Edgington T. S. Interaction of factor VIII antigen in hemophilic plasmas with human antibodies to factor VIII. J Clin Invest. 1977 May;59(5):984–989. doi: 10.1172/JCI108721. [DOI] [PMC free article] [PubMed] [Google Scholar]