Abstract
The crystal-induced chemotactic factor, a cell-derived chemoattractant for neutrophils, binds specifically to a site on human neutrophils but not to erythrocytes or lymphocytes, suggesting a relationship between the presence of specific binding sites on the neutrophils and the ability to be chemotactically activated. The Scatchard analysis revealed an equilibrium dissociation constant at 37 degrees C of 0.446 micrometer and the presence of approximately equal to 6.44 x 10(5) binding sites for 125I-crystal-induced chemotactic factor per cell. Binding was not displaced by the synthetic chemotactic factors F-Met-Leu-Phe and Gly-His-Gly or by complement-activated plasma providing evidence of the specificity of the receptor.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aswanikumar S., Corcoran B., Schiffmann E., Day A. R., Freer R. J., Showell H. J., Becker E. L. Demonstration of a receptor on rabbit neutrophils for chemotactic peptides. Biochem Biophys Res Commun. 1977 Jan 24;74(2):810–817. doi: 10.1016/0006-291x(77)90375-8. [DOI] [PubMed] [Google Scholar]
- Becker E. L., Showell H. J., Henson P. M., Hsu L. S. The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness. J Immunol. 1974 Jun;112(6):2047–2054. [PubMed] [Google Scholar]
- Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
- David G. S., Reisfeld R. A. Protein iodination with solid state lactoperoxidase. Biochemistry. 1974 Feb 26;13(5):1014–1021. doi: 10.1021/bi00702a028. [DOI] [PubMed] [Google Scholar]
- Mesibov R., Ordal G. W., Adler J. The range of attractant concentrations for bacterial chemotaxis and the threshold and size of response over this range. Weber law and related phenomena. J Gen Physiol. 1973 Aug;62(2):203–223. doi: 10.1085/jgp.62.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spilberg I., Gallacher A., Mandell B. Calcium pyrophosphate dihydrate (CPPD) crystal-induced chemotactic factor: subcellular localization, role of protein synthesis and phagocytosis. J Lab Clin Med. 1977 Apr;89(4):817–822. [PubMed] [Google Scholar]
- Spilberg I., Mandell B., Mehta J., Sullivan T., Simchowitz L. Dissociation of the neutrophill functions of exocytosis and chemotaxis. J Lab Clin Med. 1978 Aug;92(2):297–302. [PubMed] [Google Scholar]
- Spilberg I., Rosenberg D., Mandell B. Induction of arthritis by purified cell-derived chemotactic factor: role of chemotaxis and vascular permeability. J Clin Invest. 1977 Mar;59(3):582–585. doi: 10.1172/JCI108674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spilbert I., Gallacher A., Mehta J. M., Mandell B. Urate crystal-induced chemotactic factor: isolation and partial characterization. J Clin Invest. 1976 Oct;58(4):815–819. doi: 10.1172/JCI108533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward P. A., Unanue E. R., Goralnick S. J., Schreiner G. F. Chemotaxis of rat lymphocytes. J Immunol. 1977 Aug;119(2):416–421. [PubMed] [Google Scholar]
- Williams L. T., Snyderman R., Pike M. C., Lefkowitz R. J. Specific receptor sites for chemotactic peptides on human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1204–1208. doi: 10.1073/pnas.74.3.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zigmond S. H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J Cell Biol. 1977 Nov;75(2 Pt 1):606–616. doi: 10.1083/jcb.75.2.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zigmond S. H. Chemotaxis by polymorphonuclear leukocytes. J Cell Biol. 1978 May;77(2):269–287. doi: 10.1083/jcb.77.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]